1470 nm半导体激光对离体动物组织的汽化消融、切割和凝固作用  

Effects of 1470 nm Semiconductor Laser on Vaporization Ablation,Cutting,and Coagulation in Ex Vivo Animal Tissue

作　　者：郑国 潘永明[2,3] 黄俊杰 张辉 郁晨 陈民利 徐庆丰[1] 黄恒 ZHENG Guo;PAN Yongming;HUANG Junjie;ZHANG Hui;YU Chen;CHEN Minli;XU Qingfeng;HUANG Heng(Hefei Dazhu Curestar Laser Equipment Co.,Ltd.,Hefei 230088,China;Laboratory Animal Research Center/Comparative Medical Research Institute,Zhejiang Chinese Medical University,Hangzhou 310053,China;Hangzhou Lifutai Biotechnology Co.,Ltd.,Hangzhou 310051,China)

机构地区：[1]合肥大族科瑞达激光设备有限公司,合肥230088 [2]浙江中医药大学动物实验研究中心/比较医学研究所,杭州310053 [3]杭州利孚泰生物科技有限公司,杭州310051

出　　处：《实验动物与比较医学》2024年第3期279-288,共10页Laboratory Animal and Comparative Medicine

摘　　要：目的观察1470 nm波长的半导体激光对离体动物组织的汽化切割、凝固和热损伤情况,以探讨其应用于前列腺增生治疗的可行性。方法实验组和同类对照组分别使用HANS-D1型和ML-DD01FI型1470 nm半导体激光治疗仪。取新鲜离体猪膀胱组织,光纤距离组织0.5 cm和1 cm的条件下工作5 s,观察60、90、120、150、160 W功率的半导体激光对组织的损伤影响;取离体犬前列腺和猪肾组织分别进行汽化消融和汽化切割,观察60、90、120、150、160 W功率的半导体激光对组织的汽化和热损伤作用;另在凝结模式下照射离体猪肾组织5、10、15 s后,观察30、40、50 W功率的半导体激光对组织的凝固作用。结果光纤照射距离组织1 cm时,1470 nm半导体激光对邻近正常膀胱组织不会产生意外损伤;但在距离0.5 cm时,120、150、160 W的1470 nm半导体激光对膀胱组织有轻微的损伤。另外,随着输出功率的增大,60~160 W半导体激光对犬前列腺组织的汽化消融效率逐渐提高,且汽化量和消耗总能量之间呈良好的线性相关(P<0.001)。病理组织学HE染色结果显示实验组的凝固层厚度为292.20~309.98μm,汽化层深度为1.49~4.52 mm;同类对照组的凝固层厚度为289.91~303.53μm,汽化层深度为1.88~4.43 mm,两组间比较均未见明显差异(P>0.05)。同时,汽化切割截面积为1 cm2的离体猪肾组织时,60~160 W的1470 nm半导体激光对肾组织的汽化切割效率随输出功率的增大而提高(P<0.05),其中实验组的凝固层厚度为496.04~514.47μm,同类对照组的凝固层厚度为489.39~518.53μm。此外,凝结模式下30、40、50 W的半导体激光对离体猪肾组织照射5、10、15 s时,随着激光输出功率的增加,凝固瘢直径、凹槽深度和凝固效率均逐渐增大(P<0.05);其中实验组和同类对照组的凝固层厚度分别为399.10~449.98μm和392.97~447.65μm,汽化层深度分别为3.05~7.09 mm和2.70~7.14 mm,两组间比较均未见明显差异(P>0.05)Objective To observe the effects of a 1470 nm semiconductor laser on vaporization cutting,coagulation,and thermal injury of ex vivo animal tissues,aiming to explore the feasibility of its application in the treatment of benign prostatic hyperplasia.Methods The experimental group and control group were treated with HANS-D1 and ML-DD01FI 1470 nm semiconductor laser therapy equipment,respectively.Fresh ex vivo pig bladder tissue was exposed to lasers with the optical fiber placed at distances of 0.5 cm and 1 cm from the tissue for 5 s.The effects of layers at powers of 60,90,120,150,and 160 W on tissue injury were observed.Ex vivo dog prostate and pig kidney tissues were used for vaporization ablation and cutting to observe the effects of lasers at the same power levels on tissue vaporization and cutting thermal injury.Additionally,in coagulation mode,the effects of 30,40,and 50 W semiconductor lasers on tissue coagulation were observed after irradiating ex vivo pig kidney tissue for 5,10,and 15 seconds.Results When the optical fiber was placed 1 cm away from the tissue,the 1470 nm semiconductor lasers did not cause accidental damage to adjacent normal bladder tissue.However,at a distance of 0.5 cm,the 120 W,150 W,or 160 W lasers caused slight damage to the bladder tissue.In addition,with the increase in output power,the vaporization ablation efficiency of 60-160 W lasers on dog prostate tissue gradually increased,showing a good linear correlation between vaporization volume and total energy consumption(P<0.001).Histopathological HE staining results indicated that the coagulation layer thickness in the experimental group was 292.20-309.98μm,and the vaporization layer depth was 1.49-4.52 mm.In the control group,the coagulation layer thickness was 289.91-303.53μm,and the vaporization layer depth was 1.88-4.43 mm.There was no significant difference between the two groups(P>0.05).Moreover,when performing vaporization cutting on ex vivo pig kidney tissue with a cross-sectional area of 1 cm2,the efficiency of vaporizati

关 键 词：前列腺增生 1470 nm半导体激光 汽化切割 凝固 热损伤 离体动物组织 

分 类 号：R-332[医药卫生] R-605