甘氨酸对睡眠剥夺小鼠痛阈值及抑郁样行为的影响  

Effect of glycine on pain threshold and depressive-like behavior in sleep deprived mice

作　　者：向宇豪 兰淑巾 杭倩如 武玉清 XIANG Yuhao;LAN Shujin;HANG Qianru;WU Yuqing(Jiangsu Province Key Laboratory of Anesthesiology,Xuzhou Medical University,Xuzhou,Jiangsu 221004,China)

机构地区：[1]徐州医科大学江苏省麻醉学重点实验室,江苏徐州221004

出　　处：《徐州医科大学学报》2024年第4期250-253,共4页Journal of Xuzhou Medical University

基　　金：江苏省高校自然科学基金重大项目(23KJA320009);江苏高校品牌专业建设工程资助项目(PPZY2015A066)。

摘　　要：目的采用行为学实验探究甘氨酸对睡眠剥夺小鼠痛阈值及抑郁样行为的影响。方法将40只小鼠随机分为4组(n=10):对照+生理盐水组(Control+NS组)、对照+甘氨酸组(Control+Gly组)、睡眠剥夺+生理盐水组(SD+NS组)、睡眠剥夺+甘氨酸组(SD+Gly组)。采用改良多平台法建立睡眠剥夺模型。测定各组小鼠的体重;采用热板法测定各组小鼠的痛阈值;采用强迫游泳实验测定各组小鼠的抑郁样行为。结果①与Control+NS组比较,SD+NS组在第3—5天体重明显下降;SD+Gly组在第3—4天体重明显下降;②与Control+NS组比较,Control+Gly组在第5天痛阈值明显增高,SD+NS组在第5天痛阈值显著降低;与SD+NS组比较,SD+Gly组第5天痛阈值明显增高;③与Control+NS组比较,SD+NS组在第5天漂浮不动时间占总时长的百分比显著增加,SD+Gly组在第5天漂浮不动时间占总时长的百分比显著增加;与SD+NS组比较,SD+Gly组在第5天漂浮不动时间占总时长的百分比明显减少。结论甘氨酸可以提高睡眠剥夺小鼠的痛阈值,并可以缓解睡眠剥夺引起的抑郁样行为。Objective To investigate the effect of glycine on pain threshold and depressive-like behavior in sleepdeprived mice through behavioral experiments.Methods A total of 40 mice were randomly divided into four groups(n=10):a control+normal saline(Control+NS)group,a control+glycine(Control+Gly)group,a sleep deprivation+normal saline(SD+NS)group,and a sleep deprivation+glycine(SD+Gly)group.A sleep deprivation model was established using the modified multiple platform method.The body weights of the mice in each group were measured.The pain thresholds of the mice in each group were determined by the hot plate test.Their depression-like behaviors were evaluated by the forced-swimming test.Results①Compared with the Control+NS group,the SD+NS group showed significantly reduced body weight on days 3-5,while the SD+Gly group presented remarkable decreases body weight on days 3-4.②Compared with the Control+NS group,the pain threshold of the Control+Gly group significantly increased on day 5,while that of the SD+NS group remarkably decreased on day 5.Compared with the SD+NS group,the SD+Gly group had obvious increases in pain threshold on day 5.③Compared with the Control+NS group,the percentages of floating immobility time to total time on day 5 significantly increased in the SD+NS group and the SD+Gly group.Compared with the SD+NS group,the percentage of floating immobility time to total time significantly decreased in the SD+Gly group on day 5.Conclusions Glycine can increase the pain threshold of sleep deprived mice and alleviate depressive-like behavior caused by sleep deprivation.

关 键 词：甘氨酸 睡眠剥夺 痛阈 抑郁样行为 热板法 强迫游泳实验 

分 类 号：R364.5[医药卫生—病理学]