促红细胞生成素对大鼠牙髓损伤后修复性牙本质形成的促进作用研究  

Study on Promoting Effect of Erythropoietin on Restorative Dentin Formation after Dental Pulp Injury in Rats

作　　者：汪莉 钟素兰 朗么磋 张义林 WANG Li;ZHONG Sulan;LANG Mocuo;ZHANG Yilin(Department of Stomatology,the First People's Hospital of Shuangliu District,Chengdu 610200,Sichuan,China;Department of Stomatology,Stomatological Hospital of Southern Medical University,Guangzhou 510280,Guangdong,China)

机构地区：[1]成都市双流区第一人民医院口腔科,四川成都610200 [2]广州南方医科大学口腔医院口腔科,广东广州510280

出　　处：《中国美容医学》2024年第7期65-70,共6页Chinese Journal of Aesthetic Medicine

摘　　要：目的:探究促红细胞生成素(Erythropoietin,EPO)对大鼠牙髓损伤后修复性牙本质形成的作用。方法:分离并鉴定人牙髓细胞(Dental pulp cells,DPCs),将DPCs分为对照(control)组、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)组、TNF-α+EPO组;使用CCK-8法、Transwell法、碱性磷酸酶染色和茜素红染色法检测细胞增殖、迁移、成骨分化能力。将30只大鼠随机分为磷酸缓冲盐溶液(Phosphate buffered saline,PBS)组、氢氧化钙[Ca(OH)2]组和EPO组,对大鼠上颌第一磨牙进行牙髓暴露,分别以含PBS、Ca(OH)2和EPO的胶原蛋白海绵盖髓;术后1个月处死大鼠,micro-CT和苏木精-伊红染色观察修复性牙本质形成;免疫组化染色和Western blot检测牙本质基质蛋白-1(Dentin matrix protein-1,DMP-1)、牙本质涎磷蛋白(Dentin sialophosphoprotein,DSPP)、Runt相关转录因子2(Runt related transcription factor 2,Runx2)、Osterix(Osx)水平。结果:与control组相比,TNF-α组DPCs增殖、迁移、成骨分化能力降低(P<0.05);与TNF-α组相比,TNF-α+EPO组DPCs增殖、迁移、成骨分化能力提高(P<0.05)。与PBS组、Ca(OH)2组相比,EPO组露髓处形成连续完整的钙化桥及大量修复性牙本质,DMP-1、DSPP、Runx2、Osx水平升高(P<0.05)。结论:EPO通过提高DMP-1、DSPP、Runx2、Osx水平促进牙髓损伤大鼠修复性牙本质形成。Objective To explore the effect of erythropoietin(EPO)on reparative dentin formation in rats after dental pulp injury.Methods Human dental pulp cells(DPCs)was isolated and identified,and DPCs was divided into control group,tumor necrosis factor-α(TNF-α)group,TNF-α+EPO group.CCK-8 method,Transwell method,ALP staining,and alizarin red staining were used to detect cell proliferation,migration,and osteogenic differentiation.30 rats were randomly divided into PBS group,Ca(OH)2 group,and EPO group.The pulp of the maxillaryfirst molar of the rats was exposed and covered with collagen sponge containing PBS,Ca(OH)2,and EPO respectively.The rats were killed 1 month after surgery,Micro-CT and HE staining was used to observe restorative dentin formation.Immunohistochemical staining and Western blot were used to detect dentin matrix protein-1(DMP-1),dentin sialophosphoprotein(DSPP),Runt related transcription factor 2(Runx2)and Osx levels.Results Compared with control group,the proliferation,migration,and osteogenic differentiation abilities of DPCs in TNF-αgroup were decreased(P<0.05).Compared with TNF-αgroup,the proliferation,migration,and osteogenic differentiation abilities of DPCs in TNF-α+EPO group were increased(P<0.05).Compared with PBS group and Ca(OH)2 group,continuous and complete calcified bridges and a large amount of restorative dentin were formed at the pulp exposure point in EPO group,and DMP-1,DSPP,Runx2,and Osx levels were increased(P<0.05).Conclusion EPO promotes reparative dentin formation in rats with pulp injury by increasing DMP-1,DSPP,Runx2,and Osx levels.

关 键 词：促红细胞生成素 牙髓损伤修复 修复性牙本质形成 牙本质基质蛋白-1 牙本质涎磷蛋白 

分 类 号：R781[医药卫生—口腔医学]