基于网络药理学和分子对接技术探讨苗药血人参促慢性创面愈合机制  

Exploring the Mechanism of the Miao Medicine Indigofera stachyodes to Promote Chronic Wound Healing Based on Network Pharmacology and Molecular Docking Technique

作　　者：宋选飞 谢欢 周斌 刘耀 杨芳芳 徐剑 张永萍 SONG Xuanfei;XIE Huan;ZHOU Bin;LIU Yao;YANG Fangfang;XU Jian;ZHANG Yongping(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Guizhou Ethnic Medicine Transdermal Drug Delivery Engineering Technology Research Center,Guiyang 550025,China;National Engineering Research Center of Miao's Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学药学院,贵州贵阳550025 [2]贵州省民族药经皮给药制剂工程技术研究中心,贵州贵阳550025 [3]国家苗药工程技术研究中心,贵州贵阳550025

出　　处：《现代中药研究与实践》2024年第2期23-28,共6页Research and Practice on Chinese Medicines

基　　金：贵州省高等学校中药民族药(苗药)新剂型新制剂工程研究中心(黔教技[2022]022);国家苗药工程技术研究中心能力提升(黔科合中引地[2023]006)。

摘　　要：目的研究血人参多成分、多靶点及多通路治疗慢性创面愈合作用机制。方法运用网络药理学和分子对接技术筛选血人参治疗慢性创面的可能关键成分和核心靶点,并结合体内药效实验验证血人参石油醚部位的促创愈合效果。结果网络药理学筛选出38个活性成分、9个核心靶点、20个GO注释和6条KEGG通路且活性成分与核心靶点亲和力良好,体内药效结果显示血人参能通过减少炎性细胞浸润,促进细胞增殖和毛细血管的新生以缩短愈合周期。结论血人参石油醚部位可通过多成分、多靶点和多途径对慢性创面发挥治疗作用。Objective To study the mechanism of multi-component,multi-target and multi-pathway healing of chronic wounds by Indigofera stachyodes.Methods Network pharmacology and molecular docking techniques were applied to screen the possible key components and core targets of Indigofera stachyodes for the treatment of chronic wounds,and in vivo pharmacodynamic experiments were combined to validate the healing-promoting effect of Indigofera stachyodes at petroleum ether sites.Results Network pharmacological screening identified 38 active ingredients,9 core targets,20 GO annotations and 6 KEGG pathways with good affinity between the active ingredients and the core targets,and in vivo eficacy results showed that Indigofera stachyodes could shorten the healing cycle by decreasing the infiltration of inflammatory cells,and promoting the proliferation of cells and the neo-naturation of capillaries.Conclusion Indigofera stachyodes petroleum ether sites can exert therapeutic effects on chronic wounds through multi-component,multi-target and multi-travelling routes.

关 键 词：血人参 网络药理学 分子对接 慢性创面 

分 类 号：R285.5[医药卫生—中药学]