甲型流感病毒分子诊断技术的研究进展  

Research progress on molecular diagnostic technology for influenza A virus

作　　者：梁梦洁[1] 李永鑫(综述) 张新(审校)[1] LIANG Mengjie;LI Yongxin;ZHANG Xin(Department of Clinical Laboratory,Hospital of Xinjiang Production and Construction Corps/the Second Affiliated Hospital of Shihezi University School of Medicine,Urumqi,Xinjiang 830002,China)

机构地区：[1]新疆生产建设兵团医院/石河子大学医学院第二附属医院检验科,新疆乌鲁木齐830002

出　　处：《国际检验医学杂志》2024年第13期1640-1644,1650,共6页International Journal of Laboratory Medicine

基　　金：国家临床重点专科建设项目(兵财社[2023]16号);兵团医学检验临床医学研究中心建设项目(兵科发[2023]12号);兵团重点领域科技攻关计划项目(2022AB030);新疆生产建设兵团医院院级科技计划项目(2022014)。

摘　　要：甲型流感病毒(IAV)是引起急性呼吸道疾病的重要病原体之一,易引起散发大流行,对人类健康造成严重的威胁。IAV的高频变异导致精准治疗面临诸多挑战。快速、精准地识别IAV可以减少非必要的抗病毒药物使用,缩短患者病程,利于患者预后。该文主要阐述了目前检测IAV的分子诊断技术,包括核酸扩增技术、测序技术、微流控芯片技术和质谱技术,旨在根据这些技术各自的特点探讨快速、高效检测的技术手段。Influenza A virus(IAV)is one of the most important pathogens causing acute respiratory diseases,which may easily cause occasional pandemics and seriously endanger human health.Precision therapy encounters numerous challenges due to the high-frequency variation of IAV.The rapid and accurate identification of IAV could help reduce the unnecessary use of antiviral drugs,shorten the duration of patients′illness,and improve patient prognosis.This article mainly explains the current molecular diagnostic technology of IAV detection,including nucleic acid amplification technology,sequencing technology,microfluidic chip technology and mass spectrometry technology.These technologies′features are taken into consideration while discussing the technological methods for quick and effective detection.

关 键 词：甲型流感病毒 分子诊断 基因测序 

分 类 号：R446.5[医药卫生—诊断学]