TRPC1与BK-α的表达对大鼠糖尿病肾病的影响  

Exploring the Effect of TRPC1 and BK-α expression on Diabetic Nephropathy

作　　者：刘红明[1] 陈志松 邹立芳 杨智雄 喻卓[1] 胡伟[1] LIU Hongming;CHEN Zhisong;ZOU Lifang;YANG Zhixiong;YU Zhuo;HU Wei(The 2nd Department of Cardiology,the 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China)

机构地区：[1]昆明医科大学第一附属医院心脏内二科,云南昆明650032

出　　处：《昆明医科大学学报》2024年第6期15-21,共7页Journal of Kunming Medical University

基　　金：云南省科技厅基础研究计划基金资助项目(202001AY070001-184);云南省心脑血管临床医学研究中心子课题基金资助项目(ZX2019-03-01);云南省放射与治疗临床医学研究中心子课题基金资助项目(202102AA100067)。

摘　　要：目的 探究瞬时受体电位C1(transient receptor potential channel 1,TRPC1)蛋白和大电导钙离子激活钾通道α亚单位(large conductance Ca^(2+)-activated K^(+)channel α subunit,BK-α)蛋白对大鼠糖尿病肾病(diabetic kidney disease,DKD)的影响。方法 将SD大鼠随机分为对照组(n=15)和模型组(n=15)。利用高脂饲料和链脲佐菌素(streptozocin,STZ)构建DKD模型。采用血糖分析仪检测大鼠血糖变化;采用全自动生化分析仪检测大鼠肾功能水平;HE染色检测肾组织的病理变化以确定造模成功。实时荧光定量PCR(RT-qPCR)和蛋白免疫印迹分别检测肾组织TRPC1和BK-α的mRNA和蛋白表达水平;免疫组化检测TRPC1和BK-α的分布和表达情况。结果 模型组大鼠空腹血糖(fasting plasma glucose,FPG)、尿白蛋白排泄率(urinary albumin excretion rates,UAER)、血尿素氮(blood urea nitrogen,BUN)和肌酐(creatinine,Cr)均显著高于对照组(P <0.01);模型组大鼠肾小管内壁细胞出现膨胀现象,部分细胞脱离;可见肾小管发生病变或死亡;此外,在许多肾小管及肾间质区域发现有中性白细胞及其残骸;以上HE染色结果提示,DKD模型复制成功。TRPC1和BK-α在肾小球部位最为丰富,且模型组大鼠肾组织中TRPC1和BK-α的mRNA和蛋白水平都显著高于对照组(P <0.05)。结论 大鼠糖尿病肾病影响TRPC1和BK-α在肾组织中的分布和表达。Objective To explore the effect of transient receptor potential channel 1(TRPC1) protein and large conductivity Ca~(2+) activated K~+ channel α subunit(BK-α) protein on diabetic nephropathy(DKD).Methods SD rats were randomly divided into a control group(n=15) and a model group(n=15).Construct a DKD model using high-fat feed and streptozocin(STZ).Using a blood glucose analyzer to detect changes in blood glucose levels in rats;Using a fully automatic biochemical analyzer to detect the renal function level of rats;HE staining was used to detect pathological changes in renal tissue to confirm successful modeling.Real time fluorescence quantitative PCR(RT-qPCR) and Western blotting were used to detect the mRNA and protein expression levels of TRPC1 and BK-αin renal tissue,respectively;Immunohistochemical detection of the distribution and expression of TRPC1 and BK-α.Results The fasting plasma glucose(FPG),urinary albumin excretion rate(UAER),blood urea nitrogen( BUN),and creatinine(Cr) in the model group were significantly higher than those in the control group(P < 0.01);the inner wall cells of the renal tubules in the model group showed swelling,with some cells detached;visible renal tubular lesions or death.In addition,neutrophils and their debris were found in many renal tubules and interstitial regions;the above HE staining results indicate that the DKD model has been successfully replicated.TRPC1 and BK-α were most abundant in the glomerulus,and the mRNA and protein levels of TRPC1 and BK-α in the renal tissue of the model group rats were significantly higher than those in the control group(P < 0.05).Conclusion Diabetic nephropathy affects the distribution and expression of TRPC1 and BK-α in renal tissue.

关 键 词：大鼠糖尿病肾病 瞬时受体电位C1蛋白 大电导钙离子激活钾通道α亚单位蛋白 

分 类 号：R361.2[医药卫生—病理学]