小球藻生长因子对UVA诱导人皮肤成纤维细胞紫外损伤的保护作用研究  

Protective effect of Chlorella growth factor on UVA induced ultraviolet damage of human skin fibroblasts

作　　者：何亚玲 苏桂惠 唐振洲 苏志维 HE Yaling;SU Guihui;TANG Zhenzhou;SU Zhiwei(Institute of Marine Drugs,Guangxi University of Chinese Medicine,Guangxi Key Laboratory ofMarine Drugs,Nanning 530200)

机构地区：[1]广西中医药大学海洋药物研究院,广西海洋药物重点实验室,南宁530200

出　　处：《中国食品添加剂》2024年第6期86-92,共7页China Food Additives

基　　金：广西科技计划项目重点研发项目(桂科AB19259010);广西中医药大学“桂派杏林青年英才”项目(2022C039);国家自然科学基金项目(32060098);广西中医药大学海洋药物研究院团队科研专项(2018ZD005-A08)。

摘　　要：本研究通过苯酚-硫酸法、考马斯亮蓝法、紫外分光光度法对小球藻生长因子(Chlorella growth factor,CGF)中各营养成分进行含量测定;采用MTT法和酶联免疫吸附(ELISA)法分别测定CGF对紫外损伤的人皮肤成纤维细胞(HSF)的保护作用和胶原蛋白相关因子的表达。结果表明核苷酸在CGF中占比为84.40%±0.73%。CGF预处理能显著减轻UVA对HSF细胞的增殖抑制作用,与模型组相比以50μg/mL浓度CGF处理可使HSF细胞活力提高75.05%,且在5~50μg/mL内存在一定的剂量依赖关系。ELISA实验结果发现CGF可提高HSF细胞中基质金属蛋白酶抑制酶-1(TIMP-1)、I型胶原蛋白(COL-I)的表达,降低基质金属蛋白酶-1(MMP-1)的表达。CGF是优异的外源性核苷酸营养物质,能有效减轻UVA引起的HSF细胞损伤,其作用可能与促进胶原蛋白的合成有关。The nutrients composition of Chlorella growth factor(CGF)was determined by phenol-sulfuric acid method,Coomassie brilliant blue method and ultraviolet spectrophotometry.The protective effect of CGF on ultraviolet-injured human skin fibroblasts(HSF)was determined by MTT method and the expression of collagenrelated factors were determined by enzyme-linked immunosorbent(ELISA),respectively.The results showed that nucleic acids accounted for 84.40%±0.73%of CGF.Pretreatment with Chlorella Growth Factor significantly reduced the proliferation inhibition of HSF cell by UVA.Compared with the model group,treatment with a 50μg/mL concentration of CGF increased HSF cell viability by 75.05%,showing a dose-dependent relationship within 5~50μg/mL of CGF.ELISA results revealed that Chlorella growth factor could increase the expression of tissue inhibitors of metalloproteinase-1(TIMP-1)and type I collagen(COL-I)in HSF cells and reduce the expression of matrix metalloproteinase-1(MMP-1).Chlorella growth factor is an excellent exogenous nucleotide nutrient that can effectively alleviate the damage caused by UVA exposure,possibly through promoting collagen synthesis.

关 键 词：小球藻生长因子 核苷酸含量 人皮肤成纤维细胞 紫外损伤模型 Ⅰ型胶原蛋白 

分 类 号：TS254.5[轻工技术与工程—水产品加工及贮藏工程]