氯喹对白念珠菌生物膜及其耐药性的作用研究  

Effect of chloroquine on Candida albicans biofilms and its drug resistance

作　　者：吴翘楚 史般若 缪昊宸 魏昕[1] WU Qiaochu;SHI Banruo;MIAO Haochen;WEI Xin(Department of Conservative Dentistry and Endodontics,Affiliated Hospital of Stomatology,Nanjing Medical University,State Key Laboratory Cultivation Base of Research,Prevention and Treatment for Oral Diseases(Nanjing Medical University),Jiangsu Province Engineering Research Center of Stomatological Translational Medicine,Nanjing 210029,China)

机构地区：[1]南京医科大学附属口腔医学院牙体牙髓科,口腔疾病研究与防治国家级重点实验室培育建设点(南京医科大学),江苏省口腔转化医学工程研究中心,江苏南京210029

出　　处：《口腔医学》2024年第6期408-413,共6页Stomatology

基　　金：国家自然科学基金(81371156);江苏省科教能力提升工程——江苏省研究型医院(YJXYYJSDW4),江苏省医学创新中心(CXZX202227)。

摘　　要：目的探究氯喹单独使用及联用传统抗真菌药物对白念珠菌生物膜及其耐药性的改变。方法采用白念珠菌标准株及耐药株进行实验,使用XTT减低法和棋盘稀释法分别检测氯喹单独使用及联用抗真菌药物后对两种白念珠菌生物膜的抑制作用,绘制时间-杀菌曲线,扫描电镜观察两种白念珠菌生物膜形态。结果50μmol/L及以上浓度氯喹对两种白念珠菌生物膜有直接抑制作用,且随着浓度增加,抑制作用增强。氯喹与两性霉素B联用存在协同抑菌作用。氯喹单独使用和联用两性霉素B的时间-杀菌曲线结果显示,白念珠菌在不同时相培养中生长趋势有所不同;形态学观察发现均可降低白念珠菌菌丝和生物膜形成能力。结论氯喹对白念珠菌生物膜存在直接抑制,可降低白念珠菌生物膜耐药性。氯喹与两性霉素B联用时具有协同作用。Objective To evaluate the effects of chloroquine alone and in combination with traditional antifungal agents on the Candida albicans biofilms and its drug resistance.Methods This study used standard strains of Candida albicans,and drug-resistant strains of Candida albicans.The inhibitory effects of chloroquine alone and in combination with antifungal drugs on biofilms of Candida albicans were detected by XTT reduction method and chessboard dilution method respectively.The morphological characteristics of biofilms were observed under scanning electron microscopy.Results Chloroquine at the concentration of 50μmol/L or above showed a direct inhibitory effect and increased with concentration.Chloroquine combined with amphotericin B had a synergistic inhibitory effect.Results of the time-killing curve showed that the growth trends of biofilms treated with chloroquine alone and combined with amphotericin B varied in different time periods during the experimental culture.Morphological observation also revealed that chloroquine alone and in combination with amphotericin B could reduce the ability of Candida albicans to form hyphae and biofilms.Conclusion Chloroquine has an inhibitory effect on Candida albicans biofilms and can reduce its drug resistance.Furthermore,chloroquine shows a synergistic anti-fungal effect when combined with amphotericin B.

关 键 词：白念珠菌 抗真菌药物 氯喹 

分 类 号：R781.5[医药卫生—口腔医学]