银纳米粒子涂层根管镍钛器械的抗菌性能及生物相容性研究  

Study on the antibacterial performance and biocompatibility of silver nanoparticals-coated root canal nickel titanium instruments

作　　者：金泓 王汇文 吴雨婷 戴明睿 冷迪雅 朱婷婷 吴大明 JIN Hong;WANG Huiwen;WU Yuting;DAI Mingrui;LENG Diya;ZHU Tingting;WU Daming(Department of Endodontics,The Affiliated Stomatological Hospital of Nanjing Medical University,State Key Laboratory Cultivation Base of Research,Prevention and Treatment for Oral Diseases(Nanjing Medical University),Jiangsu Province Engineering Research Center of Stomatological Translational Medicine,Nanjing 210029,China)

机构地区：[1]南京医科大学附属口腔医院牙体牙髓病科,口腔疾病研究与防治国家级重点实验室培育建设点(南京医科大学),江苏省口腔转化医学工程研究中心,江苏南京210029

出　　处：《口腔医学》2024年第6期438-442,共5页Stomatology

基　　金：江苏省科教能力提升工程——江苏省研究型医院(YJXYYJSDW4);江苏省医学创新中心(CXZX202227)。

摘　　要：目的探讨银纳米粒子涂层根管镍钛器械(silver nanoparticals-coated root canal nickel titanium instruments,AgNPs-NiTi)的抗菌性能及生物相容性。方法利用脉冲电化学沉积技术制备AgNPs-NiTi。场发射扫描电镜观察AgNPs-NiTi的形貌,X射线衍射仪和能谱仪分析涂层的元素组成及含量,万能材料实验机检测AgNPs-NiTi的机械性能。将AgNPs-NiTi与粪肠球菌(E.faecalis)悬液共培养,细菌菌落计数法检测AgNPs-NiTi对E.faecalis的抗菌效果。构建离体牙根管内E.faecalis生物膜模型,将AgNPs-NiTi放入根管内模拟器械分离,用场发射电镜、活死菌染色法观察AgNPs-NiTi对E.faecalis生物膜的抗菌效果。AgNPs-NiTi与Raw264.7细胞共培养,用CCK-8法检测其细胞毒性。结果脉冲电化学沉积法在NiTi器械上构建银纳米粒子涂层,载银后NiTi器械的机械性能无明显变化。AgNPs-NiTi能够明显抑制E.faecalis的增殖,并显著破坏根管内E.faecalis生物膜。AgNPs-NiTi对Raw264.7的增殖无明显影响,无明显细胞毒性。结论AgNPs-NiTi的机械性能与镍钛器械相仿,可抑制E.faecalis及其生物膜的活性,具有良好的生物相容性。Objective To investigate the antibacterial performance and biocompatibility of silver nanoparticles-coated root canal nickel titanium instruments(AgNPs-NiTi).Methods AgNPs-NiTi was prepared using pulse electrochemical deposition.The morphology of AgNPs-NiTi was observed using field emission scanning electron microscopy(FE-SEM),and the elemental composition and content were analyzed using X-ray diffraction(XRD)and energy dispersive spectroscopy(EDS).The mechanical properties of AgNPs-NiTi were tested.After Co-culturing AgNPs-NiTi with E.faecalis,the antibacterial effect was detected by colony-forming units method.By constructing an in vitro model of E.faecalis biofilm in the root canal of teeth,the antibacterial effect of AgNPs-NiTi was observed using FE-SEM and live/dead bacterial staining.In addition,AgNPs-NiTi was co-cultured with Raw 264.7 cells,and its cytotoxicity was detected by CCK-8.Results The pulse electrochemical deposition was used to construct a silver nanoparticle(AgNPs)coating on NiTi instruments with no significant change in the mechanical properties.AgNPs-NiTi significantly inhibited the proliferation of E.faecalis and damaged E.faecalis biofilm in the root canal.AgNPs-NiTi had no significant influence on the proliferation of Raw264.7 cells and had no cytotoxicity.Conclusion The mechanical properties of AgNPs-NiTi are similar to those of nickel titanium instruments.AgNPs-NiTi inhibits E.faecalis proliferation with good biocompatibility.

关 键 词：根管镍钛器械 银纳米粒子涂层 抗菌 生物相容性 

分 类 号：R783.1[医药卫生—口腔医学]