电子支气管镜联合SHOX2和RASSF1A甲基化检测对早期肺癌的诊断价值  

Diagnostic value of electronic bronchoscopy combined with SHOX2 and RASSF1A methylation detection for early lung cancer

作　　者：林亚聪 崔清云 LIN Ya-Cong;CUI Qing-Yun(Department of Respiratory and Critical Care Medicine,Maoming People′s Hospital,Maoming 525000,Guangdong,China)

机构地区：[1]茂名市人民医院呼吸与危重症医学科,广东茂名525000

出　　处：《吉林医学》2024年第7期1591-1593,共3页Jilin Medical Journal

基　　金：茂名市科技项目[项目编号:220415114550966]。

摘　　要：目的:浅析早期肺癌采取电子支气管镜联合SHOX2和RASSF1A甲基化检测的临床效果,以便为日后临床制定检测方案提供参考。方法:抽取2022年11月~2023年4月茂名市人民医院28例早期肺癌患者行电子支气管镜细胞学及病理活检检查,同时对患者的血浆、痰液及支气管肺泡灌洗液三种标本采用甲基化特异性方式检测SHOX2和RASSF1A甲基化表达。结果:电子支气管镜确诊阳性检出率为67.86%,SHOX2和RASSF1A基因甲基化检测灌洗液检出阳性率较高,分别为71.43%、60.71%;电子支气管镜+SHOX2、RASSFIA甲基化联合检查,准确度、阳性预测、阴性预测、敏感度及特异性均高于其他检测方式。结论:电子支气管镜检查联合血浆、痰液及支气管肺泡灌洗液HOX2和RASSF1A甲基化检测能提高早期肺癌的检出率,对肺癌的早期诊断有临床应用价值。Objective To analyze the clinical effect of electronic bronchoscopy combined with SHOX2 and RASSF1A methylation detection for early lung cancer,so as to provide a reference for future clinical development of detection scheme.Method Twenty eight patients with early lung cancer from November 2022 to April 2023 were selected for electronic bronchoscopy cytology and pathological biopsy.At the same time,the methylation specific expression of SHOX2 and RASSF1A was detected in three samples of patients′plasma,sputum and bronchoalveolar lavage fluid.Results The positive rate of diagnosis by electronic bronchoscopy was 67.86%.The positive rates of SHOX2 and RASSF1A gene methylation detection lavage were relatively high,which were 71.43%and 60.71%,respectively;The accuracy,positive prediction,negative prediction,sensitivity and specificity of electronic bronchoscopy+SHOX2,RASSFIA methylation combined examination were higher than those of other detection methods.Conclusion Electronic bronchoscopy combined with HOX2 and RASSF1A methylation detection in plasma,sputum and bronchoalveolar lavage fluid can improve the detection rate of early lung cancer,and has clinical application value for early diagnosis of lung cancer.

关 键 词：早期肺癌 SHOX2 RASSF1A 电子支气管镜 诊断 

分 类 号：R734.2[医药卫生—肿瘤]