菌株JB18的鉴定及对菊苣幼苗生理指标的影响  

Identification of strain JB18 and its effect on physiological indices of chicory

作　　者：苑新悦 刘宇恒 李春月 刘博[1] 刘丽杰[1,2] 秦祥宇 于保刚 李铁[1,2] 金忠民[1,2] YUAN Xinyue;LIU Yuheng;LI Chunyue;LIU Bo;LIU Lijie;QIN Xiangyu;YU Baogang;LI Tie;JIN Zhongmin(School of Life Sciences,Agriculture and Forestry,Qiqihar University,Qiqihar 161006,China;Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihar University,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]齐齐哈尔大学抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,黑龙江齐齐哈尔161006

出　　处：《高师理科学刊》2024年第6期81-86,共6页Journal of Science of Teachers＇College and University

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(NO.145309523)。

摘　　要：铅镉污染严重危害植物生长与人类健康.筛选了一株铅镉抗性细菌JB18,进行了16SrDNA序列分析鉴定.用不同铅镉复合质量浓度处理土壤并种植菊苣,探究JB18对菊苣幼苗生理指标的影响.测定了菊苣的可溶性糖、可溶性蛋白、谷胱甘肽、脯氨酸的质量分数变化.结果表明,经鉴定菌株JB18为耐辐射甲基杆菌(Methylobacterium radiotolerans),其最大耐铅镉质量浓度为Pb-Cd(200/40)mg·L^(-1),能够水解淀粉,利用麦芽糖.盆栽实验表明,接种JB18后,可提升菊苣幼苗的可溶性糖、可溶性蛋白、脯氨酸等物质的质量分数,同时可促进谷胱甘肽的合成,减缓铅镉胁迫对菊苣幼苗的不利影响.Lead and cadmium pollution seriously jeopardizes plant growth and human health.A strain of Pb-Cdresistant bacterium JB18 was screened and identified by 16SrDNA sequence analysis.The soil was treated with different Pb-Cd composite concentrations and planted with chicory to investigate the effects of JB18 on some physiological indexes of chicory seedlings.Changes in the mass fraction of soluble sugars,soluble proteins,glutathione,and proline were determined in chicory.The results showed that JB18 was identified as a Methylobacterium radiotolerans with a maximum resistance to lead and cadmium concentration of Pb-Cd(200/40)mg·L^(-1),and was able to hydrolyze starch and utilize maltose.The pot experiment showed that inoculation with JB18 could enhance the mass fraction of soluble sugar,soluble protein,proline and other substances in chicory seedlings,

关 键 词：铅 镉 菊苣 耐辐射甲基杆菌 

分 类 号：Q94[生物学—植物学]