骨碎补总黄酮双水相提取工艺优化及抗氧化活性研究  

Optimisation of Aqueous Two-Phase Extraction Process and Antioxidant Activities of Total Flavonoids from Drynariae Rhizoma

作　　者：钱慧琴 方李珂 唐丽杰 张青瑾 张宵悦 李彦灵 QIAN Huiqin;FANG Like;TANG Lijie;ZHANG Qingjin;ZHANG Xiaoyue;LI Yanling(Sanquan College of Xinxiang Medical University,Henan Xinxiang 453003,China;Xinxiang Medical University,Henan Xinxiang 453003,China)

机构地区：[1]新乡医学院三全学院,河南新乡453003 [2]新乡医学院,河南新乡453003

出　　处：《饲料工业》2024年第13期120-128,共9页Feed Industry

基　　金：河南省科技攻关项目[222102310374];河南省高等学校重点科研项目[23B360007]。

摘　　要：试验旨在优化超声辅助PEG400-(NH_(4))_(2)SO_(4)双水相体系提取骨碎补中总黄酮的工艺,并对骨碎补中总黄酮的抗氧化活性进行评价。在单因素试验的基础上,以(NH_(4))_(2)SO_(4)质量分数、PEG400质量分数、超声功率以及超声时间为自变量,以骨碎补中总黄酮得率为因变量,采取响应面法优化骨碎补中总黄酮的提取工艺。以维生素C为阳性对照,分别测定骨碎补总黄酮对1,1-二苯基-2-苦基肼自由基(1,1-Diphenyl-2-picryl-hydrazyl radical,DPPH·)、羟基自由基(Hydroxyl radical,·OH)的清除能力和铁还原力。结果表明:骨碎补中总黄酮的最佳提取工艺为PEG400质量分数19%,(NH_(4))_(2)SO_(4)质量分数19%,超声功率180 W,超声时间40 min,该条件下骨碎补总黄酮得率为183.29 mg/g,与理论预测值接近。抗氧化活性分析表明,在一定的浓度范围内,骨碎补总黄酮对DPPH·、·OH的清除能力和铁还原力随浓度的增加不断增强并趋于稳定。以PEG400-(NH_(4))_(2)SO_(4)双水相体系提取骨碎补中总黄酮的方法可行,且最佳工艺条件可靠。This experiment was designed to optimize the extraction process of total flavonoids from Drynariae Rhizoma(DR) in ultras oundassisted PEG400-(NH_4)_2SO_(4) aqueous two-phase system and to evaluate its antioxidant activity.Based on the single-factor approach,the optimal extraction process of total flavonoids from DR was optimized by response surface methodology with(NH_4)_2SO_(4) mass fraction,PEG400 mass fraction,ultrasonic power and ultrasonic time as independent variables and the yield of total flavonoids from DR as dependent variables.The scavenging ability of DPPH·and ·OH and the iron reducing power of total flavonoids from DR were determined by using Vitamin C as a positive control.The results showed that the optimal extraction conditions for total flavonoids from DR were PEG400 mass fraction 19%,(NH_4)_2SO_(4) mass fraction 19%,ultrasonic power 180 W,and ultrasonic time 40 min.The yield of total flavonoids from DR under this process was 182.98 mg/g,which was similar to the theoretical prediction.The analysis of antioxidant activity showed that the scavenging ability of DPPH·,·OH and iron reducing power of total flavonoids from DR increased and stabilized with the increase of concentration in a certain concentration range.The PEG400-(NH_4)_2SO_(4) aqueous twophase system was used for the extraction of total flavonoids from DR,and the optimal process conditions were reliable.

关 键 词：骨碎补 总黄酮 双水相 超声波提取 响应面法 

分 类 号：S816.7[农业科学—饲料科学]