LncRNA HOTTIP单核苷酸多态性与宫颈癌及宫颈上皮内瘤变的关系  

Correlation of lncRNA HOTTIP single nucleotide polymorphism with the occurrence and development of cervical cancer and cervical intraepithelial neoplasia

作　　者：王春桃 夏春军 梁戈玉 翟宝茜 曹雪 冯延璐 陆欣苑 辛有为 WANG Chuntao;XIA Chunjun;LIANG Geyu(Provincial Engineering Research Center Platform,Jiangsu Vocational College of Medical,Jiangsu,Yancheng 224000,China;不详)

机构地区：[1]江苏医药职业学院省工程研究中心平台,江苏省盐城市224000 [2]江苏省建湖县人民医院 [3]环境医学工程教育部重点实验室 [4]江苏省盐城市第一人民医院肿瘤科

出　　处：《河北医药》2024年第12期1765-1770,共6页Hebei Medical Journal

基　　金：中华人民共和国科学技术部外国专家项目(编号:DL2022014005L);盐城市科技计划项目(编号:YCBE202316);江苏医药职业学院自然科学基金(编号:20229104)。

摘　　要：目的 探讨HOTTIP rs1859168和rs17427960单核苷酸多态性(SNP)与宫颈癌及宫颈上皮内瘤变(CIN)发病风险的关系。方法 收集2019年11月至2021年5月盐城市第一人民医院诊断为CIN的患者142例作为CIN组,被诊断为宫颈癌的患者103例作为宫颈癌组,以同期住院的170例无妇科疾病的非肿瘤患者为对照组,采用TaqMan-MGB探针法方法进行基因分型,采用χ^(2)检验和logistic回归分析SNP位点与宫颈癌及宫颈上皮内瘤变的相关性。结果 与对照组相比,HOTTIP rs1859168和rs17427960在宫颈癌患者中的基因型分布存在显著性差异。在共显性模型中,携带rs1859168 CA基因型(ORadj=1.897,95%CI:1.090~3.300,Padj=0.023)及rs17427960 AC基因型(ORadj=1.787,95%CI:1.020~3.131,Padj=0.042)均显著增加了宫颈癌的发病风险;在显性模型中,rs1859168 CA+AA基因型(ORadj=1.690, 95%CI:1.004~2.844,Padj=0.048)增加了宫颈癌的发病风险。结论 HOTTIP rs1859168和rs17427960的SNP与宫颈癌的发病风险相关,可作为宫颈癌的潜在易感性位点。Objective To explore the correlation between long non-coding RNA(LncRNA)HOXA transcript at the distal tip(HOTTIP)rs1859168,rs17427960 single nucleotide polymorphisms(SNPs)with the risk of cervical cancer(CC)and cervical intraepithelial neoplasia(CIN)Methods A total of 14 patients diagnosed as CIN in Yancheng No.1 people’s Hospital from November 2019 to May 2021 were included in CIN group,and those diagnosed as CC were included in CC group.During the same period,170 non-cancer non-gynecological disease patients admitted in our hospital were included in control group.Genotyping was performed using the TaqMan-MGB Probe.The correlation of lncRNA HOTTIP SNPs with CC and CIN was detected by Chi-square test and logistic regression analysis.Results There were significant differences in the genotype distributions of lncRNA HOTTIP rs1859168 and rs17427960 between control group and CC group(P<0.05).In the codominant model,people carrying lncRNA HOTTIP rs1859168 CA(OR adj=1.897,95%CI 1.090-3.300,P adj=0.023)and rs17427960 AC(OR adj=1.787,95%CI 1.020-3.131,P adj=0.042)had a significantly increased risk of CC.In the dominant model,people carrying lncRNA HOTTIP rs1859168 CA+AA(OR adj=1.690,95%CI 1.004-2.844,P adj=0.048)had a significantly increased risk of CC.They were potential susceptible loci for CC.Conclusion HOTTIP rs1859168 and rs17427960 SNPs are associated with the risk of cervical cancer and they can be used as potential susceptibility loci for cervical cancer.

关 键 词：宫颈癌 宫颈上皮内瘤变 HOTTIP 单核苷酸多态性 相关性研究 

分 类 号：R737.33[医药卫生—肿瘤]