ITGB1在胃癌组织中的表达及其对免疫治疗疗效的影响  

Analysis of ITGB1 expression in gastric cancer tissues and its impact on the efficacy of immunotherapy

作　　者：乔洪源[1] 付娟[1] 赵新程 庞雅琪 QIAO Hongyuan;FU Juan;ZHAO Xincheng;PANG Yaqi(Department of Radiotherapy and Chemotherapy,Second People's Hospital of China Three Gorges University,Yichang 443000,China)

机构地区：[1]三峡大学第二人民医院肿瘤放化疗科,宜昌443000

出　　处：《免疫学杂志》2024年第2期188-194,共7页Immunological Journal

基　　金：宜昌市医疗卫生科研项目(A23-1-042)。

摘　　要：目的探索ITGB1在胃癌组织中的表达及其对免疫治疗疗效影响,为胃癌的预后判断及免疫治疗疗效预测提供新的标志物。方法通过生物信息学方法对癌症基因图谱(TCGA)数据库中胃癌患者的转录组数据、临床病理特征和生存信息进行分析,评估ITGB1在胃癌中的表达及其与临床病理特征的相关性,并进一步分析ITGB1表达与免疫浸润、免疫检查点相关基因、免疫亚型及免疫治疗响应的关系。结果ITGB1在胃癌组织中高表达,ITGB1高表达组T分期晚、存活率低,总体生存率明显降低。免疫浸润评分分析显示CD4+T细胞、CD8+T细胞、中性粒细胞、巨噬细胞和树突状细胞的在ITGB1高表达组评分升高,同时免疫检查点相关基因SIGLEC15、TIGIT、CD274、HAVCR2、CTLA4、PDCD1LG2在ITGB1高表达组表达水平升高,且ITGB1的表达与免疫检查点相关基因SIGLEC15、TIGIT、CD274、HAVCR2、CTLA4、LAG3、PDCD1LG2的表达呈正相关。TISIDB数据库分析显示ITGB1在胃癌不同免疫亚型中的表达存在差异,其中C6亚型(TGF-β主导型)中表达水平升高。TIDE算法提示ITGB1高表达组TIDE评分高于低表达组,对免疫检查点阻断治疗疗效差。结论ITGB1在胃癌组织中高表达,是胃癌患者预后差的危险因素,可作为判断胃癌预后及预测免疫治疗疗效的潜在标志物。The study aimed to investigate the expression of ITGB1 in gastric cancer tissues and its impact on the efficacy of immunotherapy,and to find a new biomarker for prognosis assessment and prediction of immunotherapy response in gastric cancer.By utilizing bioinformatics methods to analyze the transcriptomic data,clinical pathological characteristics,and survival information of gastric cancer patients in the Cancer Genome Atlas(TCGA)database,this study evaluates the expression of ITGB1 in gastric cancer and its correlation with clinical features.Furthermore,an in-depth analysis was conducted for evaluating the relationship of ITGB1 expression with immune infiltration,immune checkpoint-related genes,immune subtypes and immunotherapy response.Data showed that high ITGB1 expression in gastric cancer tissues is associated with later T stage,lower survival rates,and lower overall survival.Analysis of immune infiltration scores showed the score for CD4+T cells,CD8+T cells,neutrophils,macrophages and dendritic cells were higher in the high ITGB1 expression group.Additionally,the levels of immune checkpoint-related genes such as SIGLEC15,TIGIT,CD274,HAVCR2,CTLA4 and PDCD1LG2 were elevated in the high ITGB1 expression group,and the expression of ITGB1 was positively correlated with the expression of immune checkpoint-related genes including SIGLEC15,TIGIT,CD274,HAVCR2,CTLA4,LAG3 and PDCD1LG2.Analysis based on the data from the TISIDB database revealed differential expression of ITGB1 in various immune subtypes of gastric cancer,with significantly higher expression levels in the C6 subtype(TGF-βdominant type).The TIDE algorithm indicated a high score for the group with high ITGB1 expression,suggesting poor efficacy of immune checkpoint blockade therapy.To sum up,we find that high expression of ITGB1 in gastric cancer tissues is a poor prognostic indicator for gastric cancer patients,thus ITGB1 may serve as a potential biomarker for assessing prognosis and predicting the efficacy of immunotherapy in gastric cancer.

关 键 词：ITGB1 胃腺癌 免疫治疗 生物信息学 

分 类 号：R735.2[医药卫生—肿瘤]