基于多组分含量分析和指纹图谱的生稻芽质量评价研究  

Quality evaluation of Oryzae fructus germinatus based on multi-component content analysis and fingerprint analysis

作　　者：赵越 胡啸峰 张双双 李蕾 赵永钦 王如伟 ZHAO Yue;HU Xiaofeng;ZHANG Shuangshuang;LI Lei;ZHAO Yongqin;WANG Ruwei(College of Pharmaceutical Science,Zhejiang Chinese Medical University,Hangzhou 311400,China;Yangzijiang Pharmaceutical Group Co.,Ltd.,Taizhou 225321,Jiangsu,China)

机构地区：[1]浙江中医药大学药学院,浙江杭州311400 [2]扬子江药业集团有限公司,江苏泰州225321

出　　处：《浙江农业学报》2024年第4期760-772,共13页Acta Agriculturae Zhejiangensis

摘　　要：基于HPLC进行多成分含量测定和指纹图谱技术,结合化学模式识别法对生稻芽的质量水平进行评价,为生稻芽后续深入研究与开发提供依据。采用HPLC法建立18批不同批次生稻芽指纹图谱,通过对照品指认出生稻芽中3种主要酚酸类成分,并测定了样品中3种酚酸的含量。通过中药色谱指纹图谱相似度评价系统(2012版)和IBM SPSS Statistics 23进行相似度评价、聚类分析、主成分分析和因子载荷分析。18批生稻芽指纹图谱共标定了6个共有峰,指认的3个共有峰为香草酸、对香豆酸和阿魏酸,指纹图谱相似度范围为0.868~0.995,具有较好的一致性。样品中香草酸、对香豆酸和阿魏酸含量分别为16.70~54.10μg·g^(-1)、27.00~49.54μg·g^(-1)、23.09~50.81μg·g^(-1)。聚类分析将样品分为3类,18批样品中16批聚为一类,来自浙江杭州(S14)、浙江杭州(S17)的样品分别聚为一类,主成分分析用两个主成分进行综合评价,综合得分大于1的前2位分别为S14、S17,18批样品中3种酚酸成分的总含量与主成分分析综合得分存在明显的相关性。建立的生稻芽质量评价方法操作简便、结果可靠,香草酸、对香豆酸和阿魏酸3个主要酚酸可以作为生稻芽质量评价的指标性成分。Based on HPLC multi-component content determination and fingerprint technology,combined with chemical pattern recognition method,the quality level of Oryzae fructus germinatus was evaluated,which provided a basis for further research and development of Oryzae fructus germinatus.The fingerprints of 18 batches of Oryzae fructus germinatus were established by HPLC.Three main phenolic acids in Oryzae fructus germinatus were identified by reference substances,and the contents of three phenolic acids in samples were determined.Similarity evaluation,cluster analysis,principal component analysis and factor loading analysis were performed by the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Edition).A total of 6 common peaks were identified in the fingerprints of 18 batches of Oryzae fructus germinatus,and 3 common peaks were identified as vanillic acid,p-coumaric acid and ferulic acid.The similarity of the fingerprints ranged from 0.868 to 0.995,with good consistency.The contents of vanillic acid,p-coumaric acid and ferulic acid in the samples were 16.70-54.10μg·g^(-1),27.00-49.54μg·g^(-1) and 23.09-50.81μg·g^(-1),respectively.The samples were divided into three categories by cluster analysis,16 batches of 18 batches of samples were clustered into one category,the samples of Hangzhou(S14)in Zhejiang and Hangzhou(S17)in Zhejiang were clustered into one category respectively.The principal component analysis was evaluated by two principal components.The top two comprehensive scores greater than 1 were S14 and S17,respectively.There was a significant correlation between the total content of three phenolic acids in 18 batches of samples and the comprehensive score of principal component analysis.The established quality evaluation method of Oryzae fructus germinatus is simple and reliable.Vanillic acid,p-coumaric acid and ferulic acid can be used as index components for quality evaluation of Oryzae fructus germinatus.

关 键 词：生稻芽 含量测定 香草酸 对香豆酸 阿魏酸 指纹图谱 

分 类 号：S567[农业科学—中草药栽培]