蒸发光散射检测器高效液相色谱法同时测定猪肉中4种氨基糖苷类抗生素  

Determination of 4 aminoglycosides in pork by high performance liquid chromatography with evaporative light scattering detector method

作　　者：肖雪 商广勇 马岩堂 张鹏 王子言 XIAO Xue;SHANG Guangyong;MA Yantang;ZHANG Peng;WANG Ziyan(Inspur Yunzhou Industrial Internet Co.,Ltd.,Jinan 250101,China;Inspur(Shandong)Quality Chain Technology Co.,Ltd.,Jinan 250101,China)

机构地区：[1]浪潮云洲工业互联网有限公司,济南250101 [2]浪潮(山东)质量链科技有限公司,济南250101

出　　处：《化学分析计量》2024年第6期68-72,共5页Chemical Analysis And Meterage

摘　　要：建立蒸发光散射检测器高效液相色谱法(HPLC-ELSD)测定猪肉中妥布霉素、安普霉素、卡那霉素、新霉素4种氨基糖苷类抗生素残留。在固液比1∶10下,采取磷酸盐缓冲液(pH值为7.0)提取猪肉样品中残留的氨基糖苷类抗生素,通过SupelMIP^(TM) SPE-Aminoglycosides分子印记固相萃取柱净化,利用HPLC-ELSD法检测提取液中4种目标物的含量。4种氨基糖苷类抗生素的质量浓度为0.1~10μg/mL范围内与对应的色谱峰面积线性关系良好,相关系数分别为0.992~0.999 4。4种目标物的平均回收率为87.2%~97.3%,测定结果的相对标准偏差为5.25%~8.68%(n=6)。该方法操作简便,灵敏度高,结果准确,满足猪肉中妥布霉素、安普霉素、卡那霉素和新霉素的残留检测要求。A high performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD)method was developed for the determination of four aminoglycoside antibiotics residues in pork,including tobramycin,apramycin,kanamycin and neomycin.The residues of aminoglycosides in the pork samples were extracted with phosphate acid buffer solution in ratio of solid to liquid which was 1/10.Through SupelMIP^(TM) SPE-Aminoglycosides cartridge kept the analytes bound to the sorbent,so that the extract was purified.Subsequently,the target objects were determined by HPLC-ELSD method.The mass concentration of the four aminoglycoside antibiotics had a good linear relationship with the corresponding peak area in the range of 0.1-10μg/mL,and the correlation coefficients were 0.992-0.9994..The average recoveries ranged from 87.2%to 97.3%at three spiked levels in pork,and the relative standard deviations of determination results were 5.25%-8.68%(n=6).The method is simple,sensitive and accurate,and meets the requirements for the detection of tobramycin,apramycin,kanamycin and neomycin residues in pork.

关 键 词：蒸发光散射检测器高效液相色谱法 猪肉 氨基糖苷类抗生素 残留 

分 类 号：O657.7[理学—分析化学]