降低lncRNA-RMRP表达抑制人肺癌细胞系A549的增殖和侵袭  

Decrease of lncRNA⁃RMRP expression inhibits proliferation and invasion of human lung cancer cell line A549

作　　者：郑红[1] 陈晓霞[1] 韩李周[1] 陈苗[1] 皇甫娟[1] ZHENG Hong;CHEN Xiaoxia;HAN Lizhou;CHEN Miao;HUANGFU Juan(Department of Respiratory and Critical Care Medicine,Jiaozuo People′s Hospital,Jiaozuo 454000,China)

机构地区：[1]河南省焦作市人民医院呼吸与危重症医学科,河南焦作454000

出　　处：《基础医学与临床》2024年第7期974-978,共5页Basic and Clinical Medicine

基　　金：2020年河南省科技攻关项目(LHGJ20200835)。

摘　　要：目的探讨抑制线粒体RNA加工核糖核酸内切酶RNA组分(RMRP)表达对人肺癌细胞系A549增殖和侵袭的影响;检测非小细胞肺癌(NSCLC)患者全血和组织中RMRP表达,比较在不同临床指标表达差异性,以期为NSCLC机制研究提供参考资料。方法收集2016年3月至2021年3年在焦作市人民医院行手术治疗的NSCLC患者122例,同期,在体检中心选取健康者50名作为对照组。RT-qPCR检测全血和组织中RMRP mRNA水平。培养A549细胞分为si-RMRP组、siRNA-NC组和空白组(blank组)。RT-qPCR、CCK-8法和Transwell小室法分别检测细胞中RMRP表达、增殖活性和侵袭细胞数。结果NSCLC患者全血中RMRP相对表达量显著高于对照组(P<0.001);NSCLC组织中RMRP相对表达量高于癌旁组织(P<0.001);低分化、淋巴结转移和TNM分期Ⅲ的NSCLC患者全血和组织中RMRP相对表达量较高分化、未发生淋巴结转化和TNM分期Ⅰ~Ⅱ明显升高(P<0.05);Si-RMRP组细胞中RMRP相对表达量低于空白组(blank组)和siRNA-NC组(P<0.001);相比与blank组和siRNA-NC组,24、48、72和96 h时si-RMRP组细胞吸光度(A)值均降低(P<0.05);Si-RMRP组细胞侵袭数低于blank组和siRNA-NC组(P<0.001)。结论NSCLC患者全血和组织中RMRP相对表达量升高,下调A549细胞中RMRP基因表达可抑制细胞增殖,减少细胞侵袭。Objective To investigate the effect of inhibiting the expression of RNA component of mitochondrial RNA processing endoribonuclease(RMRP)on the proliferation and invasion of human lung cancer cell line A549 in order to provide evidence to support the research on NSCLC mechanism.Methods A total of 122 cases of patients who underwent surgical treatment were selected in Jiaozuo People′s Hospital from March 2016 to March 2021.In the same period,50 healthy people from physical examination center were selected as the control group.RT⁃qPCR was used to detect the expressions of RMRP in blood and tissues.Human lung cancer cell line A549 was cultured and divided into si⁃RMRP group,siRNA⁃NC group and blank group.RT⁃qPCR,CCK⁃8 and Transwell assays were used to detect the expression of RMRP,proliferation activity and cell counting of invasive cells.Results The rel⁃ative expression level of RMRP in the blood of NSCLC patients was significantly higher than that of control group(P<0.001).The relative expression level of RMRP in NSCLC tissues was significantly higher than the adjacent tissues(P<0.001).The relative expression level of RMRP in blood and tissues of patients with poorly differenti⁃ated,lymph node metastasis and TNM stageⅢcompared with moderate to highly differentiated,no lymph node metastasis and TNM stageⅠ-Ⅱwere significantly increased(P<0.05).The relative expression level of RMRP in the cells of the si⁃RMRP group was lower than that of blank and the siRNA⁃NC group(P<0.001).Compared with the blank group and the siRNA⁃NC group,the absorbance(A)value of cells at 24,48,72 and 96 h in the si⁃RMRP group was decreased(P<0.05).The number of invasion cells in the si⁃RMRP group was lower than that in the blank group and the siRNA⁃NC group(F=27.765,P<0.001).Conclusions The relative expression levels of RMRP in blood and tissues of NSCLC patients are increased.Down⁃regulation of the expres⁃sion of RMRP gene in A549 cells can inhibit cell proliferation and reduce cell invasion.

关 键 词：非小细胞肺癌 长链非编码RNA(lnRNA) 线粒体RNA加工核糖核酸内切酶RNA组分(RMRP) 增殖 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]