环状RNA编码蛋白在疾病发生机制中的作用研究进展  

Progress of research on circular RNA encoding proteins in disease

作　　者：焦娇 徐丹丹 孔岩[1] 张志珺[2,3] Jiao Jiao;Xu Dandan;Kong Yan;Zhang Zhijun(School of Medicine,Southeastern University,Nanjing,Jiangsu 210009,China;Institute of Neuropsychiatry,Department of Neurology,Zhongda Hospital of Southeastern University,Nanjing,Jiangsu 210009,China;Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,Guangdong 518055,China)

机构地区：[1]东南大学医学院,南京210009 [2]东南大学附属中大医院神经内科神经精神研究所,南京210009 [3]中国科学院深圳先进技术研究院,深圳518055

出　　处：《中华医学遗传学杂志》2024年第7期872-880,共9页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金(82130042)。

摘　　要：随着研究的进展,非编码RNA被发现突破了传统的定义,可通过编码序列元件,与核糖体结合,直接编码功能性蛋白。circRNA编码蛋白的功能在非编码RNA中研究最为广泛。本研究以"circRNA"、"encode"和"translation"作为关键词,对PubMed以及Web of Science数据库进行检索,对文献进行筛选和引用检索,对circRNA的翻译机制、相关研究方法以及与疾病的相关性进行综述。circRNA可通过非帽依赖途径翻译蛋白质。多种分子技术、尤其是蛋白质谱分析对circRNA编码蛋白独特肽段的鉴定,对于证实circRNA编码蛋白的存在具有重要的价值。CircRNA编码蛋白参与消化、神经、泌尿、乳腺等多个系统疾病的调控,具有成为疾病诊断和治疗新靶标的潜力。本文对circRNA翻译领域的理论基础、现有实验方法和疾病相关研究进行了全面的归纳,为后续的诊断和治疗靶标开发提供了线索。With the advance of research,non-coding RNA has been found to surpass the traditional definition to directly code functional proteins by coding sequence elements and binding with ribosomes.Among the non-coding RNAs,the function of circRNA encoded proteins has been most extensively studied.This study has used"circRNA","encoded",and"translation"as the key words to search the PubMed and Web of Science databases.The retrieved literature was screened and traced,with the translation mechanism,related research methods,and correlation with diseases of circRNA reviewed.CircRNA can translate proteins through a non-cap-dependent pathway.Multiple molecular techniques,in particular mass spectrometry analysis,have important value in identifying unique peptide segments of circRNA encoded proteins for confirming their existence.The proteins encoded by the circRNA are involved in the pathogenesis of diseases of the digestive,neurological,urinary systems and the breast,and have the potential to serve as novel targets for disease diagnosis and treatment.This article has provided a comprehensive review for the basic theory,experimental methods,and disease-related research in the field of circRNA translation,which may provide clues for the identification of new diagnostic and therapeutic targets.

关 键 词：环状RNA 翻译蛋白 疾病 

分 类 号：R363[医药卫生—病理学]