三七白及粉对脑出血型应激性溃疡大鼠胃黏膜的保护作用及其对NRLP3介导的细胞焦亡途径的影响  

Protective effect of panax notoginseng white powder on stress ulcer induced by ratcerebral hemorrhage and its effect on NRLP3-mediated pyroptosis pathway

作　　者：安凯 郭沛然 孙玉凤[3] 贾舒雅 An Kai;Guo Peiran;SunYufeng;Jia Shuya(Department of Rehabilitation Medicine,Hebei Shijiazhuang People's Hospital,Shijiazhuang 050000,China)

机构地区：[1]石家庄市人民医院康复医学二科,河北050000 [2]河北医科大学中西医结合学院 [3]河北医科大学第二医院消化内科

出　　处：《脑与神经疾病杂志》2024年第7期434-437,共4页Journal of Brain and Nervous Diseases

基　　金：河北省中医药管理局项目(2019454)。

摘　　要：目的探讨三七白及粉对脑出血型应激性溃疡(SU)大鼠胃黏膜的保护作用及机制。方法采用自体血定位注射法建立大鼠基底核脑出血SU模型。受试大鼠分为3组:对照组、模型组、三七白及粉组。免疫组化染色分析溃疡指数并观察胃组织病理变化。试剂盒检测大鼠血清氧化氮(NO)、丙二醛(MDA)、超氧化物歧化酶(SOD)、前列腺素E2(PGE2)的水平。Western blot分析NLRP3抗原介导的焦亡相关蛋白(NLRP3、ASC、cleaved caspase-1和N-GSDMD)以及促炎细胞因子(IL-1β和IL-18)的蛋白水平。结果三七白及粉对大鼠脑出血型SU模型有良好的促进愈合作用,三七白及粉治疗可以提高脑出血型SU模型大鼠血清NO、SOD和PGE2量含并降低血清中的MDA水平。此外,三七白及粉治疗阻断了与NLRP3介导的焦亡相关蛋白(NLRP3、ASC、cleaved caspase-1和N-GSDMD)的上调以及炎症因子(IL-1β和IL-18)的蛋白水平。结论三七白及粉可以保护脑出血型SU大鼠胃黏膜损伤,其可能通过是阻断NRLP3介导的细胞焦亡,增强抗氧化应激作用,进而增强胃黏膜的保护作用。Objective To investigate the protective effect of panax notoginseng white powder on gastric mucosa of rats with cerebral hemorrhage stress ulcer(SU)and its possible mechanism.Methods The stress ulcer model of rat basal ganglia hemorrhage was established by autologous blood injection.The tested rats were divided into three groups:control group,model group,panax notoginseng white powder groups.HE staining was used to analyze ulcer index and observe gastric histopathologic changes.The serum levels of nitric oxide(NO),malondialdehyde(MDA),Superoxide Dismutase(SOD)and Prostaglandin E2(PGE2)were detected by the kit.Western blot analysis of N1RP3-mediated caustic death associated proteins(NLRP3,ASC,cleaved caspase-1 and N-GSDMD)and pro-inflammatory cytokines(IL-1βand IL-18)protein levels.Results Panax Notoginseng white powder and powder have a good effect on promoting healing of cerebral hemorrhage SU model.Panax Notoginseng white powder and powder can increase serum NO,SOD and PGE2 content and decrease serum MDA level in cerebral hemorrhage SU model rats.In addition,Notochiwhite and powder treatment blocked the upregulation of NLRP3-mediated pyro proteins(NLRP3,ASC,cleaved caspase-1,and N-GSDMD)and the protein levels of inflammatory factors(IL-1βand IL-18).Conclusion Panax notoginseng white and powder can protect gastric mucosa damage in rats with cerebral hemorrhage stress ulcer,which may enhance the protective effect of gastric mucosa by blocking NRLP3-mediated pyrodeath and enhancing the antioxidant stress effect.

关 键 词：三七白及粉 脑出血 应激性溃疡 NRLP3 细胞焦亡 

分 类 号：R734.34[医药卫生—肿瘤]