利用非产溶剂梭菌合成生物丁醇研究进展  

Recent advances on the synthesis of biobutanol by non-solvent microorganisms

作　　者：于乐永 于海瑞[1] 赵有志 王利华[1] 袁紫乙 庄文静 YU Leyong;YU Hairui;ZHAO Youzhi;WANG Lihua;YUAN Ziyi;ZHUANG Wenjing(Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong(Weifang University),Weifang Key Laboratory of Coho Salmon Culturing Facility Engineering,Institute of Modern Facility Fisheries,College of Biology and Oceanography,Weifang University,Weifang 261061,China;Weifang Centre for the Promotion of Scientific and Technological Innovation,Weifang 261000,China)

机构地区：[1]生物化学与分子生物学山东省高校重点实验室(潍坊学院),潍坊市银鲑养殖设施工程重点实验室,现代设施渔业研究院,潍坊学院生物与海洋学院,山东潍坊261061 [2]潍坊市科技创新促进中心,山东潍坊261000

出　　处：《食品与发酵工业》2024年第13期323-330,共8页Food and Fermentation Industries

基　　金：山东省重大科技创新工程项目(2018CXGC0102,2019JZZY020710)。

摘　　要：丁醇作为一种重要的大宗化学品和潜在的生物燃料在很多领域都有广泛的应用。化石燃料的枯竭和合成生物技术的进步重新引起了人们对生物丁醇生产的兴趣。传统的生物丁醇可以由产溶剂梭菌发酵多种碳源生产,其途径被称为丙酮-丁醇-乙醇途径。然而,其相对缓慢的生长速度、较低的丁醇耐受性和生产效率阻碍了其进一步应用。最近,研究者们对于一些其他有前景的工业宿主作为底盘生产丁醇进行了研究,包括酿酒酵母、大肠杆菌、酪丁酸梭菌等。该文全面总结了不同非产溶剂菌株用于生产丁醇的优势和挑战,以更好地确定用于生产丁醇的理想非溶剂宿主。此外,还提出了进一步提高丁醇产量的策略。As an important bulk chemical and potential biofuel,butanol has a wide range of applications in many fields.The depletion of fossil fuels and the progress of synthetic biotechnology have renewed interest in the production of biobutanol.Solvent-producing Clostridium is commonly used to produce butanol through traditional acetone-butanol-ethanol(ABE)fermentation.However,its relatively slow growth rate,low butanol tolerance and production efficiency hinder its further application.Recently,researchers have studied some other promising industrial hosts as the chassis to produce butanol,including Escherichia coli,Saccharomyces cerevisiae,C.tyrobutyricum and so on.This review comprehensively summarizes the advantages and challenges of different non-solvent strains for the production of butanol,in order to better determine the ideal non-solvent host for the production of butanol.In addition,a strategy to further increase the production of butanol was also proposed.

关 键 词：丁醇 非产溶剂微生物 丙酮-丁醇-乙醇发酵 代谢工程 

分 类 号：TQ923[轻工技术与工程—发酵工程]