不同地方品种鸡白痢阳性鸡沙门氏菌的分离及药敏试验  

Isolation and Drug Sensitivity Test of Salmonella from Different Local Chicken Breeds with Positive Pullorum

作　　者：林荣连 张业怀 凌丁 Lin Ronglian;Zhang Yehuai;Ling Ding(Shuangqiao Town Aquatic Animal Husbandry and Veterinary Station,Wuming District,Nanning City,Nanning,530104,Guangxi;Guangxi Agricultural Vocational and Technical University,Nanning,530007,Guangxi)

机构地区：[1]南宁市武鸣区双桥镇水产畜牧兽医站,广西南宁530104 [2]广西农业职业技术大学,广西南宁530007

出　　处：《中国畜禽种业》2024年第6期103-109,共7页The Chinese Livestock and Poultry Breeding

基　　金：2019年广西农业科技自筹经费项目(Z201989);2023年广西农业职业技术大学科研项目(XKJ2328)。

摘　　要：为了解广西六个地方品种鸡沙门氏菌的感染和耐药状况,从广西各地收集灵山麻鸡、广西三黄鸡、南丹瑶鸡、东兰乌鸡、容县霞烟鸡、天峨六画山鸡六个地方品种90份样品作为研究对象,每个品种选择15只临床表现健康的成年鸡进行鸡白痢血清学检测,再从检测为鸡白痢阳性鸡中随机抽取2只鸡进行剖检,采集病料分离沙门氏菌,并对分离菌株进行药物敏感试验。结果表明:检测六个地方品种90个样品,阳性样品22份,样品鸡白痢阳性率为24.44%,六个地方品种中广西三黄鸡个体阳性率最高,为46.67%;采集12个鸡白痢阳性鸡的病料,分离出4株沙门氏菌,鸡白痢阳性鸡沙门氏菌检出率为33.33%,检出率最高的鸡品种是广西三黄鸡为100%。4株分离菌药敏试验结果显示,4株分离菌均对头孢曲松、头孢哌酮、环丙沙星、氧氟沙星高度敏感,对阿莫西林、庆大霉素、四环素和新霉素具有不同程度耐药性。可见,被调查的广西六个地方品种源养殖场鸡白痢沙门氏菌感染依然存在且比较严重,而且分离菌对多种药物具有不同程度的耐药性,养殖户要根据实际加强鸡白痢净化工作,定期进行沙门氏菌的药敏性试验,选择高效敏感药物进行防控,以提高养殖效益。To understand the infection and drug resistance of Salmonella in six local breeds of chickens in Guangxi,90 samples of six local breeds of Lingshan Partridge Chicken,Guangxi Sanhuang Chicken,Nandan Yao Chicken,Donglan Wu Chicken,Rongxian Xiayan Chicken,and Tian'e Liuhua Shan Chicken were collected from all over Guangxi.Fifteen adult chickens with healthy clinical manifestations were selected for serological detection of pullorum disease in each breed, and two chickens were randomly selected from the chickens with positive detection of pullorum disease for necropsy. The diseased materials were collected to isolate Salmonella, and the drug sensitivity test was carried out on the isolated strains. The results showed that 22 positive samples were detected from 90 samples of six local varieties, and the positive rate of pullorum disease was 24.44 %. Among the six local varieties, the positive rate of Guangxi Sanhuang chicken was the highest, which was 46.67 %. Four strains of Salmonella were isolated from 12 pullorum-positive chickens. The detection rate of Salmonella in pullorum-positive chickens was 33.33 %, and the highest detection rate was 100 % in Guangxi Sanhuang chickens. The results of the drug sensitivity test showed that the four isolates were highly sensitive to ceftriaxone, cefoperazone, ciprofloxacin, and ofloxacin, and had different degrees of resistance to amoxicillin, gentamicin, tetracycline, and neomycin. It can be seen that the infection of Salmonella pullorum in six local breed source farms in Guangxi still exists and is serious, and the isolated bacteria have different degrees of drug resistance to various drugs. Farmers should strengthen the purification of chicken pullorum according to the actual situation, regularly carry out the drug sensitivity test of Salmonella, and select efficient and sensitive drugs for prevention and control, to improve breeding efficiency.

关 键 词：广西地方品种鸡 鸡白痢阳性 沙门氏菌 药敏试验 

分 类 号：S858.31[农业科学—临床兽医学]