Synthesis of chitosan oligosaccharide selenium and its antitumor activity  

作　　者：Yu-Mei Chen Yan Yu Chen Yin-Yin Li Yue Bai Yang Dong Bing-Qiang Zhang Meng-Meng Chen Xi-Feng Zhang Jing Liu 

机构地区：[1]College of Veterinary Medicine,Qingdao Agricultural University,Qingdao 266100,China [2]Qingdao Restore Biotechnology Co.,Ltd.,Qingdao 266111,China [3]Key Laboratory of Cancer and Immune Cells of Qingdao,Qingdao 266111,China [4]Analytical&Testing Center of Qingdao Agricultural University,Qingdao 266100,China

出　　处：《Traditional Medicine Research》2024年第10期10-20,共11页TMR传统医学研究

基　　金：supported by Localization of oxygen radicals and enzymes in bivalve haemocytes to Jing Liu(20230058,6602423063).

摘　　要：Background:Polysaccharides have various biological activities;the complexation of polysaccharides with trace element ions can produce synergistic effects,improving the original biological activities of sugars and trace elements.Methods:The preparation process of chitosan oligosaccharide selenium(COSSe)was optimized by the response surface method,followed by a detailed analysis of the resultant compound’s characteristics.The anti-cancer activity of COSSe was studied using the human ovarian cancer cell line SKOV3 as a cell model.Results:The prepared COSSe response surface was well predicted,indicating successful chitosan oligosaccharide binding with selenium.Response surface method analyses identified the optimal synthesis conditions for COSSe:the reaction time of 5.08 h,the reaction temperature of 71.8°C,and mass ratio(Na2SeO3:chitosan oligosaccharide)of 1.02.Under the optimal conditions,the final product,the selenium content,reached 1.302%.The results of cell experiments showed that COSSe significantly inhibited SKOV3 proliferation in a concentration-dependent manner.RNA-seq results showed that chitosan oligosaccharide and COSSe significantly modulated the expression of genes’DNA metabolic processes and cell cycle in SKOV3 cells.Gene enrichment analysis showed the inhibition of the cell cycle,and the results of flow cytometry showed that SKOV3 cells increased in the S phase and decreased in the G2/M phase,with a noted suppression in the protein expression of cyclin-dependent kinase 2(CDK2)and cyclin A1(CCNA1).Conclusion:COSSe has a stronger effect than chitosan oligosaccharide,leading to the arrest of the cell cycle in the S phase.Thus,COSSe may be an effective candidate for the treatment of ovarian cancer.

关 键 词：chitosan oligosaccharide chitosan oligosaccharide selenium ovarian cancer SKOV3 RNA-SEQ 

分 类 号：R285[医药卫生—中药学]