氨基寡糖素对宁夏枸杞的诱导抗病性及生化指标的影响  

Effects of amino-oligosaccharides on induced disease resistance and biochemical indices of wolfberry

作　　者：王荣山 李捷[1,2] 党慧梅 张煦 张生懂 张建金 李秀芬[4] WANG Rongshan;LI Jie;DANG Huimei;ZHANG Xu;ZHANG Shengdong;ZHANG Jianjin;LI Xiufen(College of Forestry,Gansu Agricultural University,Lanzhou 730070,China;Gansu Province Wolfberry Harmless Cultivation Engineering Research Center,Lanzhou 730070,China;Lüzhiyuan Ecological Construction Co.,LTD.Liangzhou District,Wuwei City,Gansu Province,Wuwei,Gansu 733000,China;Agricultural Technology Extension Center of Jingyuan County,Gansu Province,Jingyuan,Gansu 730699,China)

机构地区：[1]甘肃农业大学林学院,甘肃兰州730070 [2]甘肃省枸杞无害化栽培工程研究中心,甘肃兰州730070 [3]甘肃省武威市凉州区绿之源生态建设有限公司,甘肃武威733000 [4]甘肃省靖远县农业技术推广中心,甘肃靖远730699

出　　处：《江西农业大学学报》2024年第3期672-681,共10页Acta Agriculturae Universitatis Jiangxiensis

基　　金：甘肃省2022科技攻关项目(22CX8ND151)。

摘　　要：【目的】氨基寡糖素是一种植物免疫诱抗剂,可诱导激活植物免疫系统、促进生长,提高抗病能力。研究通过叶面喷施氨基寡糖素(AOS),观测分析枸杞病害发病情况和生理生化指标分析,阐明AOS对枸杞植株生长的影响和诱导抗病的生理机制。【方法】以4年生‘宁杞7号’为试验材料,以清水为对照,从初花期开始,间隔15 d叶面喷施5%AOS 800倍液1次,连续喷施4次,诱导32 d后调查枸杞叶斑病发病情况3次。同时采集叶片样品,测定叶片厚度、叶绿素含量、活性氧代谢途径、苯丙烷代谢途径关键酶活性和次生代谢产物含量。【结果】叶面喷施AOS诱导32 d后,‘宁杞7号’叶片叶绿素、O_(2)^(-)、H_(2)O_(2)含量达到最高,分别较对照组提高63.04%、13.27%和206.14%,抗氧化酶SOD、POD、PAL活性提升最高,较对照组提升19.56%、45.46%和48.85%,MDA含量最低,较对照组下降37.43%;诱导39 d后,‘宁杞7号’叶斑病的发病率、病情指数最低,分别为5.90%、1.66,相对防治效果最佳,达到83.02%,此时4CL活性最高,较对照组提高107.39%,次生代谢产物木质素、总酚和类黄酮含量也达到最高,分别较对照提高26.89%、63.98%和19.69%;诱导46 d后,CAT、C4H活性最高,分别较对照组提高380.02%、45.34%。【结论】叶面喷施AOS可提高‘宁杞7号’叶片叶绿素含量,能诱导产生ROS,使O_(2)^(-)、H_(2)O_(2)含量一定程度升高,同时增强SOD、POD、CAT、PAL、C4H、4CL活性,降低MDA含量,积累次生代谢产物木质素、总酚和类黄酮,显著降低叶斑病的自然发病率和病情指数。该研究结果可为氨基寡糖素在枸杞无害化栽培中的应用提供理论依据。[Objective]Amino-oligosaccharide(AOS)is a kind of plant immune attractor,which can induce and activate plant immune system,promote growth and improve disease resistance.In this study,by spraying AOS on leaf surface,the incidence of wolfberry disease and physiological and biochemical indexes were observed and analyzed,and the effects of AOS on the growth of wolfberry plants and the physiological mechanism of inducing disease resistance were clarified.[Method]In this experiment,four-year-old wolfberry‘Ningqi VII’plants were selected as experimental materials.Clean water was used as a control treatment while AOS solution was sprayed onto the foliage in the experimental group.Starting from the initial flowering period,5%AOS solution diluted 800 times was sprayed onto the leaf surface four times,once every 15 days.After 32 days of induction,the onset of barbary wolfberry leaf spot disease was investigated for a total of three times.At the same time,leaf samples were collected to determine leaf thickness,chlorophyll content,active oxygen metabolism pathway,phenylpropane metabolism pathway key enzyme activities and secondary metabolites content[.Result]The results showed that by spraying AOS on leaves for 32 days,the contents of chlorophyll,O_(2)^(-)(Superoxide anion)and H_(2)O_(2)(Hydrogen peroxide)levels of wolfberry‘Ningqi VII’reached the highest,which were increased by 63.04%,13.27%and 206.14%compared with control group,respectively.And antioxidant enzyme activity activities of SOD(superoxide dismutase),POD(peroxidase)and PAL(phenylalanine aminolyase)reached their peak values with notable increases compared to those in the control group.Specifically,there was a significant increase of 13.27%in O_(2)^(-)level,206.14%increase in H_(2)O_(2)level,19.56%increase in SOD activity,45.46%increase in POD activity,and 48.85%increase in PAL activity.The MDA content is reduced to a minimum,decreased by 37.43%compared with control group.After 39d of the induction,the incidence and disease index of‘Ningqi VII’leaf spot

关 键 词：‘宁杞7号’ 氨基寡糖素 诱导抗病 活性氧代谢 苯丙烷代谢 

分 类 号：S567[农业科学—中草药栽培]