石蒜种子萌发过程及生理研究  

Study on germination process and physiology of Lycoris radiata

作　　者：萨日娜 蔡军火[1] 游欣 温婷 姜雪茹 魏绪英[2] 钟良涛 SA Rina;CAI Junhuo;YOU Xin;WEN Ting;JIANG Xueru;WEI Xuying;ZHONG Liangtao(College of Landscape Architecture and Art,Jiangxi Agricultural University,Nanchang 330045,China;College of Art,Jiangxi University of Finance and Economics,Nanchang 330032,China)

机构地区：[1]江西农业大学园林与艺术学院,江西南昌330045 [2]江西财经大学艺术学院,江西南昌330032

出　　处：《江西农业大学学报》2024年第3期682-690,共9页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(32360420、31960327);江西省花卉产业技术体系专项(JXARS-17)。

摘　　要：【目的】探究石蒜种子萌发过程中的形态与生理变化规律,为其优质种苗的繁育提供理论基础及高效繁殖技术。【方法】以成熟新鲜的石蒜种子为研究材料,在观测其萌发第0、7、14、21、28、35天形态变化的基础上,采用考马斯亮蓝G-250法、蒽酮比色法、酶联免疫法测定种子内的可溶性蛋白质、可溶性糖和淀粉、内源激素(GA_(3)、ABA、IAA、ZR)等内含物。【结果】(1)石蒜种子的萌发方式为子叶留土型,依据形态变化特征,可将其萌发过程划分为未萌发期(0 d)、露白期(7~14 d)、子叶伸长期(8~14 d)、根快速生长期(15~21 d)、分根期(22~28 d)和真叶形成期(29~35 d)6个时期,持续时长约35 d。(2)在种子萌发过程中,淀粉、可溶性蛋白、可溶性糖、内源ABA的含量及GA_(3)/ABA比值总体均呈“下降”趋势,IAA和ZR的含量却呈“上升”趋势,GA_(3)含量则“先降后升”;其中,子叶伸长期和根快速生长期是内源激素变化的拐点时期。(3)种子的萌发进程与ZR、IAA的含量变化呈极显著正相关,而与GA_(3)、ABA的含量变化则呈显著负相关。高含量的IAA和ZR、低含量的ABA和GA_(3)有利于胚乳内淀粉的水解,从而促进种子的萌发。【结论】石蒜种子的萌发是一个动态耗能过程,主要依赖胚乳内的淀粉和可溶性糖提供能量。同时,种子的萌发也受到内源激素ZR和IAA的正向调控及GA_(3)/ABA的负向调控。[Objective]Explore the morphological and physiological changes in the germination process of Lycoris radiata seeds,and provide scientific theoretical basis and efficient reproduction technology for the breeding of high-quality seedlings.[Method]Using mature and fresh L.radiata seeds as the research material,the morphological changes were observed on days 0,7,14,21,28,and 35 during germination.Based on these observations,soluble proteins,soluble sugars and starch,endogenous hormones(GA_(3),ABA,IAA,ZR)were measured by coomassie brilliant blue G-250,anthrone colorimetry,enzyme-linked immunosorbent assay,respectively.[Result](1)The germination mode of L.radiata seeds is hypogeal germination,and based on morphological changes,the germination process can be divided into six stages:non-germination stage(0 d),germination stage(7-14 d),cotyledon elongation stage(8-14 d),rapid root growth stage(15-21 d),root separation stage(22-28 d),and true leaf formation stage(29-35 d).The entire germination process lasts approximately 35 days.(2)In the process of seed germination,the content of starch,soluble protein,soluble sugar,endogenous ABA and the GA_(3)/ABA ratio generally showed a“decrease”,while the content of endogenous IAA and ZR showed an“increase”,while the content of GA_(3)“decreased first and then increased”.Among them,cotyledon elongation and rapid root growth period are the inflection points of endogenous hormone changes.(3)There was a very significant positive correlation between the germination process and the content changes of ZR and IAA,significant negative correlation with the content change of GA_(3)and ABA.This indicates that high levels of IAA and ZR,and low levels of ABA and GA_(3)are beneficial for the hydrolysis of starch within the endosperm,thereby promoting seed germination.[Conclusion]The germination of L.radiata seeds is a dynamic energy-consuming process,which mainly relies on the starch and soluble sugars within the endosperm to provide energy.Additionally,the germination of the seeds is

关 键 词：石蒜 种子萌发 淀粉 可溶性糖 可溶性蛋白质 内源激素 

分 类 号：S682.29[农业科学—观赏园艺]