基于SIRT1/PGC-1α/Cyt C通路探讨养精种玉汤联合寿胎丸对PCOS-IR大鼠的影响  

Yangjing Zhongyu decoction combined with Shoutai pill on PCOS-IR in rats based on SIRT1/PGC-1α/Cyt C pathway

作　　者：聂春莹 马红霞[2] 胡敏 NIE Chunying;MA Hongxia;HU Min(Graduate School of Guangxi University of Chinese Medicine,Nanning 530001,Guangxi,China;The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510163,Guangdong,China)

机构地区：[1]广西中医药大学研究生院,广西南宁530001 [2]广州医科大学附属第一医院,广东广州510163

出　　处：《现代中西医结合杂志》2024年第11期1478-1484,1507,共8页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金资助项目(82374501,81973892)。

摘　　要：目的基于沉默信息调节因子2相关酶1(SIRT1)/过氧化物酶体增殖活化受体γ辅助活化因子1α(PGC-1α)/细胞色素C(Cyt C)通路探究养精种玉汤联合寿胎丸对多囊卵巢综合征合并胰岛素抵抗(PCOS-IR)大鼠的影响。方法取雌性SD大鼠110只,随机选取97只大鼠,以皮下注射脱氢表雄酮及高脂饲料喂养方法建立PCOS-IR模型,其余大鼠作为正常组。将造模成功后大鼠随机分为8组,每组12只。二甲双胍组给予0.265 g/kg二甲双胍灌胃,寿胎丸组给予12 g/kg寿胎丸灌胃,低剂量养精种玉汤组给予33 g/kg养精种玉汤灌胃,高剂量养精种玉汤组给予66 g/kg养精种玉汤灌胃,低剂量养精种玉汤+寿胎丸组给予33 g/kg养精种玉汤+12 g/kg寿胎丸灌胃,高剂量养精种玉汤+寿胎丸组给予66 g/kg养精种玉汤+12 g/kg寿胎丸灌胃,高剂量养精种玉汤+寿胎丸+EX-527组给予66 g/kg养精种玉汤+12 g/kg寿胎丸灌胃和5 mg/kg EX-527腹腔注射,正常组和模型组给予生理盐水灌胃与腹腔注射,均1次/d,连续干预14 d。记录大鼠的体重;血糖仪、ELISA试剂盒分别检测大鼠的空腹血糖(FPG)和空腹胰岛素(FINS)水平,计算胰岛素抵抗指数(HOMA-IR)和血糖曲线下面积(GAUC);ELISA法检测大鼠血清黄体生成素(LH)、卵泡刺激素(FSH)、睾酮(T)、雌二醇(E_(2))水平;HE染色观察大鼠卵巢组织病理学形态;Western blot法检测大鼠卵巢组织中SIRT1/PGC-1α/Cyt C信号通路相关蛋白表达情况。结果与正常组比较,模型组大鼠FPG、GAUC、HOMA-IR、LH、T水平及卵巢组织中Cyt C蛋白相对表达量均明显升高(P均<0.05),FSH、E_(2)水平及卵巢组织中SIRT1、PGC-1α蛋白相对表达量均明显降低(P均<0.05);大鼠卵巢组织呈多囊样病变,囊性扩张卵泡增多,卵泡体积增大,颗粒细胞排列疏松紊乱。与模型组比较,各药物组大鼠的体重下降程度较大(P均<0.05),FPG、GAUC、HOMA-IR、LH、T水平及卵巢组织中Cyt C蛋白相对表达量均明显降低Objective It is to investigate the effect of Yangjing Zhongyu decoction combined with Shoutai pill in the treatment of polycystic ovary syndrome complicated with insulin resistance(PCOS-IR)in rats based on the silent information regulation 2 homolog 1(SIRT1)/peroxisome proliferator-activated receptorγcoactivator-1α(PGC-1α)/cytochrome C(Cyt C)pathway.Methods A total of 110 female SD rats were selected,in which 97 rats were randomly selected and given DHEA by subcutaneous injection and fed with high-fat diet to establish PCOS-IR models,and the other rats were taken as normal group.After successful modeling,the rats were randomly divided into 8 groups,with 12 rats in each group.The metformin group was given metformin 0.265 g/kg by gavage,the Shoutai pill group was given Shoutai pill 12 g/kg by gavage,the low-dose and high-dose groups of Yangjing Zhongyu decoction group was respectively given Yangjingzhongyu decoction 33 g/kg and 66 g/kg by gavage,the low-dose Yangjing Zhongyu decoction+Shoutai pill group was given Yangjing Zhongyu decoction 33 g/kg and Shoutai pill 12 g/kg,the high-dose Yangjing Zhongyu decoction+Shoutai pill group was given Yangjing Zhongyu decoction 66 g/kg and Shoutai pill 12 g/kg by gavage,the high-dose Yangjing Zhongyu decoction+Shoutai pill+EX-527 group was given Yangjing Zhongyu decoction 66 g/kg,Shoutai pill 12 g/kg by gavage and EX-5275 mg/kg by intraperitoneal injection,the normal group and model group were given normal saline by intragastric injection and intraperitoneal injection,all once daily,continuously treated for 14 days.The body weights of the rats were recorded;the levels of fasting plasma glucose(FPG)and fasting insulin(FINS)of the rats were respectively determined by blood glucose meters and ELISA kits,and their insulin resistance index(HOMA-IR)and glucose area under the curve(GAUC)were calculated;the serum levels of luteinizing hormone(LH),follicle stimulating hormone(FSH),testosterone(T),and estradiol(E_(2))were determined by ELISA;the pathological changes in ovarian tissu

关 键 词：多囊卵巢综合征 胰岛素抵抗 养精种玉汤 寿胎丸 沉默信息调节因子2相关酶1 过氧化物酶体增殖活化受体γ辅助活化因子1α 细胞色素C 

分 类 号：R-332[医药卫生]