新型免疫检查点Siglec-15表达与老年结直肠癌预后相关性研究  

Association between the expression of a novel check point Siglec-15 and the prognosis of elderly patients with colorectal cancer

作　　者：丁润 许里[4] 杨俊荣 毛圆[3] 朱一丹 梁国栋 DING Run;XU Li;YANG Junrong;MAO Yuan;ZHU Yidan;LIANG Guodong(Department of General Surgery,the Fourth Affiliated Hospital of Nanjing Medical University,Nanjing 210031,China;Department of Pathology,the Fourth Affiliated Hospital of Nanjing Medical University,Nanjing 210031,China;Department of Oncology,the Fourth Affiliated Hospital of Nanjing Medical University,Nanjing 210031,China;Department of Pathology,Jiangsu Cancer Hospital,Nanjing 210009,China;Department of Gastroenterology,Geriatric Hospital of Nanjing Medical University,Nanjing 210024,China)

机构地区：[1]南京医科大学第四附属医院普外科,江苏省南京市210031 [2]南京医科大学第四附属医院病理科,江苏省南京市210031 [3]南京医科大学第四附属医院肿瘤科,江苏省南京市210031 [4]江苏省肿瘤医院病理科,江苏省南京市210009 [5]南京医科大学附属老年医院消化内科,江苏省南京市210024

出　　处：《实用老年医学》2024年第7期669-673,共5页Practical Geriatrics

基　　金：国家自然科学基金面上项目(82073389)。

摘　　要：目的 检测Siglec-15在老年结直肠癌中的表达情况及其与结直肠癌主要临床病理指标及预后的关系。方法 在6例新鲜结直肠癌及癌旁组织上行一步法定量聚合酶链式反应(qPCR)检测,在组织芯片上(60例老年结直肠癌标本及癌旁对照标本)进行免疫组化检测,使用Cox回归分析并绘制Kaplan-Meier生存曲线探讨老年结直肠癌病人预后的影响因素。结果 qPCR及免疫组化结果均显示,结直肠癌组织中的Siglec-15表达明显高于癌旁组织(P=0.002,0.019)。Siglec-15的表达与老年结直肠癌病人的性别(P=0.037)、肿瘤部位(P=0.040)、淋巴结转移(P=0.037)及TNM分期(P=0.003)相关。COX回归分析和Kaplan-Meier生存曲线表明,Siglec-15表达(HR=2.39),肿瘤分化(HR=3.81)及远处转移(HR=9.53)是老年结直肠癌病人预后的影响因素(均P<0.05)。结论 Siglec-15在结直肠癌组织中异常高表达且与老年结肠癌的临床病理特征相关。Siglec-15可作为老年结直肠癌总体生存期的预测指标。Objective To examine the expression of Sialic acid-binding immunoglobulin-like lectins(Siglec)-15 in the elderly patients with colorectal cancer(CRC)and to investigate the relationship of the expression of Siglec-15 with the clinical characteristics and the prognosis of CRC.Methods One-step quantitative polymerase chain reaction(qPCR)test was used in 6 fresh CRC samples as well as the corresponding non-cancerous tissue samples and tissue microarrays,and 60 elderly CRC samples and non-cancerous samples were detected by immunohistochemistry analysis.Cox regression and Kaplan-Meier survival analysis were conducted to evaluate the prognostic parameters in this elderly patients with CRC.Results The results of qPCR test and immunohistochemistry analysis showed that the expression of Siglec-15 in CRC tissues was significantly higher than that in paracancerous tissues(P=0.002,0.019,respectively).Moreover,the expression level of Siglec-15 protein in CRC was significantly related to gender(P=0.037),tumor location(P=0.040),lymph node metastasis(P=0.037)and TNM stage(P=0.003).Cox regression and Kaplan-Meier survival analysis showed that high Siglec-15 expression(HR=2.39),tumor differentiation(HR=3.81),distant metastasis(HR=9.53)were the influencing factors in the elderly patients with CRC(P<0.05).Conclusions Siglec-15 is highly expressed in the CRC tissue,and is correlated with clinicopathological features of CRC.Siglec-15 may serve as a novel prognostic factor for the elderly patients with CRC.

关 键 词：老年人 结直肠癌 Siglec-15 定量聚合酶链式反应 免疫组化 总生存期 

分 类 号：R735.3[医药卫生—肿瘤]