基于H19/miR-195-5p/SGK1通路分析金银花提取物对子宫内膜癌术后感染大鼠的干预效果  

Intervention effects of honeysuckle extract on post-operative infected rats with endometrial cancer based on H19/miR-195-5p/SGK1 pathway analysis

作　　者：张正宇 杨倩[2] 葛恒琳 石磊 刘红 ZHANG Zheng-yu;YANG Qian;GE Heng-lin;SHI Lei;LIU Hong(People's Hospital of Hongze District,Huai'an City,Jiangsu Province,Huai'an,Jiangsu 223100,China;不详)

机构地区：[1]江苏省淮安市洪泽区人民医院妇产科,江苏淮安223100 [2]南京医科大学第四附属医院妇产科,江苏南京210031

出　　处：《中华医院感染学杂志》2024年第11期1725-1729,共5页Chinese Journal of Nosocomiology

基　　金：江苏省自然科学基金青年基金资助项目(BK20210971)。

摘　　要：目的分析基于H19/微小RNA-195-5p(miR-195-5p)/糖皮质激素调节激酶1(SGK1)通路的金银花提取物对子宫内膜癌术后感染大鼠的干预效果.方法选取70只SPF级Wistar雌性大鼠,取其中30只进行LD35试验,确定致死量后,选取80 mg/kg的剂量进行给药及观察;剩余40只中10只作为空白组,30只建立子宫内膜癌术后感染模型,共有26只大鼠建模成功,将其分为模型组8只、药物对照组9只、金银花提取物组9只;药物对照组给予阿莫西林克拉维酸钾,金银花提取物组给予金银花提取物,空白组、模型组不做处理,干预14 d后观察大鼠变化.检测各组小鼠白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、金黄色葡萄球菌菌落数水平和H19、miR-195-5p、糖皮质激素调节激酶1(SGK1)mRNA表达量及相对表达量.结果与空白组相比,模型组、药物对照组、金银花提取物组IL-8、TNF-α、IL-1β、金黄色葡萄球菌菌落数水平、H19、SGK1 mR-NA表达量及相对表达量上升,miR-195-5p mRNA表达量及相对表达量下降(P<0.05);与模型组相比,药物对照组、金银花提取物组IL-8、TNF-α、IL-1β、金黄色葡萄球菌菌落数水平、H19、SGK1 mRNA表达量及相对表达量下降,miR-195-5p mRNA表达量及蛋白相对表达量上升(P<0.05);与药物对照组相比,金银花提取物组IL-8、TNF-α、IL-1β、金黄色葡萄球菌菌落数水平、H19、SGK1 mRNA表达量及相对表达量下降,miR-195-5p mRNA表达量及相对表达量上升(P<0.05).结论金银花提取物可减轻子宫内膜癌术后感染大鼠炎性反应,减少金黄色葡萄球菌菌落数,其机制可能与H19/miR-195-5p/SGK1得到调节有关.OBJECTIVE To analyze the intervention effect of honeysuckle extract on post-operative infected rats with endometrial cancer based on H19/MicrornA-195-5P(miR-195-5p)/glucocorticoid-regulated kinase 1(SGK1)pathway.METHODS Totally 70 SPF grade female Wistar rats were selected,and 30 of them were selected for LD35 test.After determining the lethal dose,a dose of 80 mg/kg was selected for administration and observation.10 out of the remaining 40 rats were used as the blank group,30 of them were used to establish the postoperative infection model of endometrial cancer,and a total of 26 rats were successfully modeled,and they were divided into 8 in the model group,9 in the drug-control group,and 9 in the honeysuckle extract group.Drug control group was given amoxicillin and clavulanate potassium,honeysuckle extract group was given honeysuckle extract,blank group and model group were not treated,and the changes of rats were observed after intervention for 14 days.The levels of interleukin-8(IL-8),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),Staphylococcus aureus colony number and the relative expression of H19,micrornA-195-5p(miR-195-5p),and glucocorticoid-regulated kinase 1(SGK1)mRNA expression in each group were detected.RESULTS Compared with the blank group,IL-8,TNF-α,IL-1β,Staphylococcus aureus colony number,H19 and SGK1 mRNA expression levels and relative expression levels were increased in model group,drug control group and honeysuckle extract group,while miR-195-5p mRNA expression levels and relative expression levels were decreased(P<0.05).Compared with model group,interleukin-8(IL-8),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),Staphylococcus aureus colony number,H19 and SGKl mRNA expression levels and relative expression levels in drug control group and honeylonicera extract group decreased.The mRNA expression and relative expression of miR-195-5p were increased(P<0.05).Compared with drug control group,IL-8,TNF-α,IL-1β,Staphylococcus aureus colony number,H19,SGK1 mRNA expression leve

关 键 词：金银花提取物 子宫内膜癌 感染 白细胞介素-8 肿瘤坏死因子-α 白细胞介素-1β 金黄色葡萄球菌菌落数 H19 微小RNA-195-5p 糖皮质激素调节激酶1 

分 类 号：R737.33[医药卫生—肿瘤]