油茶枯饼总黄酮提取工艺优化、含量测定及其抗氧化活性研究  

Optimization of extraction technology,content determination,and antioxidant activity of total flavonoids from Camellia oleifera cake

作　　者：董智丹 王宝林 黄氏玄庄 许琼明 杨世林[1,4] 陈晓春 潘其明[1] 高红伟 DONG Zhi-dan;WANG Bao-lin;HUANG Shi-xuan-zhuang;XU Qiong-ming;YANG Shi-lin;CHEN Xiao-chun;PAN Qi-ming;GAO Hong-wei

机构地区：[1]广西中医药大学药学院,广西南宁530200 [2]海防医药大学传统医学系,海防180000 [3]苏州大学,江苏苏州215000 [4]江西中医药大学,江西南昌330000

出　　处：《饲料研究》2024年第11期89-95,共7页Feed Research

基　　金：广西科技基地与人才专项(项目编号:桂科AD20297142)。

摘　　要：研究旨在通过响应面法优化油茶枯饼中总黄酮的超声提取工艺,比较不同产地油茶枯饼总黄酮含量及其体外抗氧化活性。试验以广西壮族自治区南宁市的油茶枯饼为原料,基于单因素试验结果,以乙醇浓度、液料比、超声时间、超声功率为影响因素,总黄酮得率为评价指标,运用Box-Behnken响应面法优化油茶枯饼中总黄酮的超声提取工艺。结果显示,乙醇浓度70%、液料比55 m L/g、超声时间45 min、功率500 W为油茶枯饼总黄酮超声提取的最佳工艺,在此条件下广西南宁市的油茶枯饼总黄酮提取率为3.44%。不同产地、批次的油茶枯饼总黄酮含量范围1.20%~3.54%,其中湖南省湘潭市的油茶枯饼总黄酮含量最高,广东省梅州市的油茶枯饼总黄酮含量最低。湖南省湘潭市油茶枯饼的1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力最高,其IC_(50)值为0.59 g/L;广西壮族自治区玉林市油茶枯饼的2,2'-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS+)自由基清除能力最高,其IC_(50)值为0.73 g/L。研究表明,试验可为挖掘油茶枯饼在畜禽饲养中的开发利用提供参考。The purpose of this study was to optimize the ultrasonic extraction technology of total flavonoids from Camellia oleifera cake by response surface methodology,and to compare the content of total flavonoids from Camellia oleifera cake from different habitats and its antioxidant activity in vitro.Based on the results of single factor experiment,the extraction process of total flavonoids from Camellia oleifera cake was optimized by Box-Behnken response surface methodology with ethanol concentration,ratio of liquid to material,ultrasonic time and ultrasonic power as influencing factors and the yield of total flavonoids as evaluation index,and the extraction process of total flavonoids from Camellia oleifera cake was optimized by using Box-Behnken response surface methodology.The results showed that ethanol concentration of 70%,liquid-material ratio 55 mL/g,ultrasonic time of 45 min,power 500 W were the optimum conditions for ultrasonic extraction of total flavonoids from Camellia oleifera cake.Under these conditions,the actual yield of total flavonoids from Camellia oleifera cake in Nanning,Guangxi was 3.44%.The content of total flavonoids in Camellia oleifera cake from different producing areas and batches ranged from 1.20%to 3.54%,among which Xiangtan City of Hu'nan Province had the highest content of total flavonoids,while Meizhou City of Guangdong Province had the lowest content of total flavonoids.The scavenging ability of DPPH radical of Camellia oleifera cake in Xiangtan City of Hu'nan Province was the highest,and its IC_(50) value was 0.59 g/L.The free radical scavenging ability of ABTS+was the highest,and its IC_(50) value was 0.73 g/L.The study indicates that the research can provide references for exploring the exploitation and utilization of Camellia oleifera cake in livestock and poultry breeding.

关 键 词：油茶枯饼 总黄酮 响应面法 抗氧化活性 

分 类 号：S816.7[农业科学—饲料科学]