皱环球盖菇菌丝体的耐热性及抗氧化酶对高温的响应  

Heat resistance in the mycelial stage of Stropharia rugosoannulata and response of antioxidant enzymes to high temperature

作　　者：焦潇颖 田景花[1,2] 高亚洁 李守勉 JIAO Xiaoying;TIAN Jinghua;GAO Yajie;LI Shoumian(School of Horticulture,Hebei Agricultural University,Baoding 071000,Hebei,China;Ministry of Education of China and Hebei Province Joint Innovation Center for Efficient Green Vegetable Industry,Baoding 071000,Hebei,China)

机构地区：[1]河北农业大学园艺学院,河北保定071000 [2]河北省绿色高效蔬菜产业省部共建协同创新中心,河北保定071000

出　　处：《菌物学报》2024年第6期105-114,共10页Mycosystema

基　　金：国家食用菌产业技术体系(CARS-20);河北省重点研发计划(20326328D);河北省食用菌产业创新团队(HBCT2023090202)。

摘　　要：本研究以皱环球盖菇单孢杂交后筛选出的100个杂交子代菌株作为试验材料,通过观测各菌株菌丝体高温胁迫后的恢复生长情况,筛选出17个菌丝体阶段耐热性强的超亲菌株,均能在44℃高温胁迫8 h后恢复生长(2个亲本在44℃高温胁迫8 h后死亡),其中4个菌株(QM2、QM15、QM46、QM83)在46℃高温胁迫8h后能恢复生长,具有更强的耐热能力。将耐热性强的菌株QM15和耐热性较差的菌株QM27作为研究对象,测定其菌丝体阶段在高温胁迫下超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、丙二醛(MDA)等抗氧化生理指标的动态变化,结果表明:2个皱环球盖菇菌株母种中的CAT、SOD活性均呈先上升后下降的趋势;QM27菌株POD活性也呈先上升后下降趋势,而耐热性强的QM15菌株POD活性在高温胁迫6h时恢复到胁迫前水平,胁迫8h时酶活性又略有回升,显著高于胁迫前。QM15菌株的3种抗氧化酶活性始终高于QM27,说明耐热性强的QM15菌株在高温胁迫下具有更强的抗氧化能力来减轻膜系统损伤。高温胁迫下3种抗氧化酶的活性变化可以作为皱环球盖菇菌株菌丝体阶段耐热性鉴定的生理指标。One hundred hybrid strains selected by monospore crossbreeding of Stropharia rugosoannulata were used as test materials.By observing the mycelial recovery growth of each strain after high temperature stress,seventeen hybrid strains were screened with stronger heat tolerance than parents in mycelial stage.After eight hours of high temperature stress at 44℃,these strains could survive,while both parents were dead.Among them,four hybrid strains(QM2,QM15,QM46,and QM83)had stronger heat tolerance and could survive after eight hours of high temperature stress at 46℃.The hybrid strain QM15 with strong heat resistance and hybrid strain QM27 with poor heat resistance were used as test materials to determine the dynamic changes of antioxidant physiological indexes related to heat resistance in the mycelial stage,including superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and malondialdehyde(MDA).The results showed that under high temperature stress the activities of CAT and SOD of the two strains in the mycelial stage increased first and then decreased.The POD activity of strain QM27 increased first and then decreased,while that of strain QM15 with strong heat resistance recovered the level prior to stress treatment after six hours of high temperature stress at 44℃,and the activity increased slightly after eight hour stress.The activities of three enzymes of strain QM15 were always higher than those of QM27.The results indicate that strain QM15 with strong heat resistance has stronger antioxidant capacity and can mitigate membrane damage under high temperature stress.The changes of activities of the three antioxidant enzymes under high temperature stress can be used as physiological indexes for identification of heat resistance in the mycelial stage of Stropharia rugosoannulata strains.

关 键 词：皱环球盖菇 杂交子代菌株 耐热性 高温胁迫 抗氧化酶 

分 类 号：S646[农业科学—蔬菜学]