Polyethylenimine-modified graphene quantum dots promote endothelial cell proliferation  

作　　者：Qirong Xu Chen Li Xiangyan Meng Xinghong Duo Yakai Feng 

机构地区：[1]School of Chemistry and Chemical Engineering,Qinghai University for Nationalities,Xining 810007,PR China [2]Key Laboratory of National Ethnic Affairs Commission of Resource Chemistry and Ecological Environment Protection on Qinghai-Tibet Plateau,Xining 810007,PR China [3]institute of Disaster and Emergency Medicine,Tianjin University,Tianjin 300072,PR China [4]School of Chemical Engineering and Technology,Tianjin University,Tianjin 300350,PR China [5]Key Laboratory of Systems Bioengineering(Ministry of Education),Tianjin University,Tianjin 300072,PR China

出　　处：《Regenerative Biomaterials》2024年第2期137-146,共10页再生生物材料（英文版）

基　　金：supported by the National Natural Science Foundation of China(NSFC 51963018);the‘Chunhui Plan’cooperative scientific research project of the Ministry of Education(Z2015049);the High-level Talents Project of Qinghai University for Nationalities(2019XJG01).

摘　　要：Endothelial cell proliferation plays an important role in angiogenesis and treatment of related diseases.The aim of this study was to evaluate the effect of polyethylenimine(PEI)-modified graphene quantum dots(GQDs)gene vectors on endothelial cell prolifera-tion.The GQDs-cationic polymer gene vectors were synthesized by amidation reaction,and used to deliver pzNF580 gene to Human umbilical vein endothelial cells(HUVECs)for promoting their proliferation.The chemical modification of GQDs can ad-just gene vectors'surface properties and charge distribution,thereby enhancing their interaction with gene molecules,which could effectively compress the pZNF580 gene.The CCK-8 assay showed that the cell viability was higher than 80%at higher vector concentration(40μg/mL),demonstrating that the GQDs-cationic polymer gene vectors and their gene complex nanoparticles(NPs)having low cytotoxicity.The results of the live/dead cell double staining assay were consistent with those of the CCK-8 assay,in which the cell viability of the A-GQDs/pZNF580(94.38±6.39%),C-GQDs-PEI-polylactic acid-co-polyacetic acid(PLGA)/pZNF580(98.65±6.60%)and N-GQDs-PEI-PLGA/pZNF580(90.08±1.60%)groups was significantly higher than that of the Lipofectamine 2000/pzNF580(71.98±3.53%)positive treatment group.The results of transfection and western blot experiments showed that the vector significantly enhanced the delivery of plasmid to HUVECs and increased the expression of pZNF580 in HUVECs.In addition,the gene NPs better promote endothelial cell migration and proliferation.The cell migration rate and proliferation ability of C-GQDs-PEI-PLGA/pZNF580 and N-GQDs-PEI-PLGA/pZNF580 treatment groupS were higher than those of Lipofectamine 2000/pDNA treatment group.Modified GQDs possess the potential to serve as efficient gene carriers.They tightly bind gene molecules through charge and other non-covalent interactions,significantly improving the effciency of gene delivery and ensuring the smooth release of genes within the cell.This innovative strategy provid

关 键 词：graphene quantum dots POLYETHYLENIMINE ZNF580 gene endothelial cell PROLIFERATION 

分 类 号：TQ127.11[化学工程—无机化工]