Analysis of Fatty Acid in Biological Samples Using Liquid Chromatography–Quadrupole-Orbitrap Mass Spectrometry Under Parallel Reaction Monitoring Mode  

作　　者：Jingxuan Yang Wenjun Guo Xiaohang Xu Liang Zhao Yajuan Xu Yang Wang 

机构地区：[1]School of Pharmaceutical Sciences,Changchun University of Chinese Medicine,Changchun,130117,China [2]Key Laboratory of Medicinal Materials,Jilin Academy of Chinese Medicine Sciences,Changchun,130021,China [3]Jilin Ginseng Academy,Changchun University of Chinese Medicine,Changchun,130117,China

出　　处：《Journal of Analysis and Testing》2024年第2期210-217,共8页分析检测（英文）

基　　金：supported by the National Natural Science Foundation of China(82104366);the Department of Science and Technology of Jilin Province(20210101426JC and 20220505047ZP);the Changchun University of Chinese Medicine(202326)。

摘　　要：Fatty acids(FAs)are an important class of functional small molecules and participate in a variety of life biological processes.This experiment proposed a liquid chromatography–quadrupole-Qrbitrap mass spectrometry(LC–Q-Orbitrap MS)method to perform FA profiling under parallel reaction monitoring(PRM)acquisition mode.The FA was derivatized by 2-dimethylaminoethylamine(DMED)to increase the ionization e fficiency and provide the characterized fragment pattern.The mass spectra obtained from full scan,multiple ion monitoring(MIM),and PRM were compared.The results showed that the protonated ion of FA+DMED–H2O was detected and the neutral loss of 45.06 Da was observed in the tandem mass spectrum.The PRM method provided the highest selectivity and sensitivity for FA detection with the help of accurate mass weight and specific neutral loss-based fragments.The method validation was performed using the FA standards and pooled quality control serum sample,which showed that the established method had good repeatability,stability,and linearity.Finally,the developed method was successfully applied to analyze the rat serum and brain tissue samples for the drug e ffi-cacy evaluation of gross saponin of Tribulus terrestris L.fruit(GSTTF)against middle cerebral artery occlusion(MCAO)-induced ischemic stroke.This method has extensive practicability and great potential in the detection of FA,especially for the analysis of samples with complex matrices.

关 键 词：Fatty acid DERIVATIZATION LC-Q-Orbitrap MS Parallel reaction monitoring Biological sample 

分 类 号：O657.63[理学—分析化学] Q503[理学—化学]