Resistance risk assessment of six pyrethroids and acephate toward the resistant adult tarnished plant bug,Lygus lineolaris  

作　　者：Yuzhe Du Shane Scheibener Yucheng Zhu Maribel Portilla Mingling Zhang 

机构地区：[1]USDA-ARS,Southern Insect Management Research Unit,Stoneville,USA [2]USDA-ARS,Pollinator Health in Southern Crop Ecosystem Research Unit,Stoneville,USA

出　　处：《Insect Science》2024年第3期792-802,共11页昆虫科学（英文版）

摘　　要：Due to rapidly developed resistance,pest management relies less on pyrethroids to control economically damaging infestations of the tarnished plant bug(TPB),Lygus lineolaris(Palisot de Beauvois)in cotton fields of Mississippi.Yet,pyrethroid resistance remains prevalent in TPB populations.This study assessed the resistance levels in adult TPB to six common pyrethroids and acephate.Resistant TBPs were collected from wild host plants in late October after harvest in the Mississippi Delta region of the United States.Based on LCso values,the field-resistant TPBs displayed higher resistance to permethrin,esfenvalerate,and bifenthrin(approximately 30 fold)and moderate resistance toα-cyhalothrin,β-cyfluthrin,5-cypermethrin,and acephate(approximately 15 fold).Further investigations showed that the inhibitors of three detoxification enzyme,triphenyl phosphate(TPP),diethyl maleate(DEM),and piperonyl butoxide(PBO)had synergistic effects on permethrin,γ-cyhalothrin,and bifenthrin in resistant TPBs.Furthermore,elevated esterase,GST,and P450 activities were significantly expressed in fieldresistant TPBs.Additionally,GST and esterase were reduced after 48 h exposure to certain pyrethroids at LCso dose.The synergistic and biochemical assays consistently indicated that P450 and esterase were involved in pyrethroid detoxification in TPBs.This study provides valuable information for the continued use of pyrethroids and acephate in controlling TPBs in cotton fields in the Mississippi Delta region of the United States.

关 键 词：cytochrome P450-monooxygenases(P450) detoxification enzyme activities ESTERASE Lygus lineolaris pyrethroids and organophosphate resistance tarnished plant bug 

分 类 号：Q96[生物学—昆虫学]