瑶药倒水莲暖风汤对顶体酶活性低下不育患者ROS、MMP的影响  

Effect of Yao medicine DaoShuilian Nuanfeng Decoction on ROS and MMP in infertile patients with lowacrosomal enzyme activity

作　　者：粟龙 文爱斌 张宁 李敏 韦周娜 SU Long;WEN Aibin;ZHANG Ning;LI Min;WEI Zhouna(Guilin Hospital of Traditional Chinese Medicine,Guilin,Guangxi 541000,China;Guilin Hospital of the Second Xiangya Hospital of Central South University,Guilin,Guangxi 541000,China)

机构地区：[1]桂林市中医医院,广西桂林541000 [2]中南大学湘雅二医院桂林医院,广西桂林541000

出　　处：《中国民族医药杂志》2024年第5期14-17,57,共5页Journal of Medicine and Pharmacy of Chinese Minorities

基　　金：广西自治区中医药管理局科研课题(GXZYC20230597)。

摘　　要：目的:从瑶医“盈亏平衡”理论探讨瑶药倒水莲暖风汤对顶体酶活性低下不育患者的精液常规参数、ROS、MMP和顶体酶的影响。方法:将156例精子顶体酶活性低下的患者随机分为两组,治疗组给予瑶药倒水莲暖风汤口服,对照组给予左卡尼汀口服溶液口服,疗程为3个月,对比治疗前后精液常规参数、ROS、MMP和顶体酶的变化。结果:治疗后,治疗组总有效率为89.74%,对照组总有效率为60.26%,治疗组的有效率显著高于对照组(P<0.05);治疗后,两组精液量、精子浓度、PR、PR+NP、NMS较治疗前均有不同程度提高(P<0.05),治疗组提高程度显著高于对照组(P<0.05);治疗后,两组ROS均显著下降(P<0.05),治疗组下降程度显著高于对照组(P<0.05);MMP、顶体酶活性较治疗前均显著提高(P<0.05),治疗组提高程度显著高于对照组(P<0.05)。结论:瑶药倒水莲暖风汤可显著改善精液量、精子浓度、前向运动精子、总活力和正常形态精子,提升精子顶体酶活性,且ROS水平明显下降,MMP水平明显增加,推测其改善精子质量和提高顶体酶活性的作用机制与精子内的ROS、MMP密切有关。Objective:Exploring the semen routine parameters of infertile patients with low acrosomal enzyme activity treated with Yao medicine Daoshuilian Nuanfeng Decoction based on the"Ying Kui equilibrium"theory of Yao medicine The effects of ROS,MMP,and acrosin.Methods:156 patients with low acrosomal enzyme activity of sperm were randomly divided into two groups.The treatment group was given Yao medicine Daoshuilian Nuanfeng Decoction orally,and the control group was given L-carnitine oral solution orally for 3 months.The changes of semen routine parameters,ROS,MMP and acrosomal enzyme before and after treatment were compared.Results:After treatment,the total effective rate was 89.74%in the treatment group and 60.26%in the control group.The curative effect of the treatment group was superior to the control group(P<0.05);After treatment,semen volume,sperm concentration,PR,PR+NP and NMS in both groups were significantly increased compared with before treatment(P<0.05),and the improvement in treatment group was significantly higher than that in control group(P<0.05).After treatment,ROS in both groups decreased significantly(P<0.05),and the decrease degree in treatment group was significantly higher than that in control group(P<0.05).MMP and acrosomal enzyme activities were significantly increased compared with those before treatment(P<0.05),and the improvement degree in treatment group was significantly higher than that in control group(P<O.05).Conclusion:Yao medicine Daoshuilian Nuanfeng Decoction can significantly improve semen volume,sperm concentration,forward moving sperm,total motility and normal morphological sperm,improve sperm acrosin activity,and ROS level is significantly decreased,MMP level is significantly increased.It is speculated that the mechanism of improving sperm quality and improving acrosin activity is closely related to ROS and MMP in sperm.

关 键 词：瑶医 倒水莲暖风汤 顶体酶活性 ROS MMP 

分 类 号：R29[医药卫生—民族医学]