白花丹素抑制非小细胞肺癌细胞增殖的研究  

The study of plumbagin on suppressing the proliferation of non-small cell lung cancer

作　　者：梁美斯 兰慧敏 于婷 李胜桥 Liang Meisi;Lan Huimin;Yu Ting;Li Shengqiao(Department of Chinese Medicine Oncology,Cancer Center,the Fifth Affiliated Hospital of Sun Yat-sen University,Guangdong Zhuhai 519000,China;Guangdong Provincial Engineering Research Center of Molecular Imaging,the Fifth Affiliated Hospital of Sun Yat-sen University,Guangdong Zhuhai 519000,China)

机构地区：[1]中山大学第五附属医院肿瘤中心中医肿瘤科,广东珠海519000 [2]广东省分子成像工程技术研究中心

出　　处：《中华介入放射学电子杂志》2024年第2期120-125,共6页Chinese Journal of Interventional Radiology：electronic edition

基　　金：广东省中医药管理局课题(20211079);珠海市科技创新局课题(2220004000322)。

摘　　要：目的探讨白花丹素抑制非小细胞肺癌(non-small cell lung cancer,NSCLC)的临床疗效。方法通过CCK-8实验检测白花丹素对LLC1细胞增殖的影响。同时构建Lewis肺癌模型,评估白花丹素对小鼠皮下肿瘤的治疗效果及其生物安全性。结果CCK-8实验结果表明,白花丹素可显著抑制LLC1细胞的增殖且呈现出时间依赖性和浓度依赖性。其作用机制与细胞凋亡和铁死亡相关。在Lewis肺癌模型中,与对照组相比,白花丹素治疗组具有更强的抗肿瘤作用。苏木精-伊红染色法结果显示,与对照组相比,白花丹素治疗后肿瘤组织内细胞核破裂增加,组织坏死面积扩大。结论细胞实验和动物实验均证实了白花丹素抑制NSCLC细胞的生长与增殖,且这种抑制作用与细胞凋亡和铁死亡相关。这为白花丹素治疗NSCLC提供了临床前依据。Objective To explore the tumour suppressing efficacy of plumbagin(PLB)on nonsmall cell lung cancer(NSCLC).Methods We used CCK-8 assay to detecte the effect of PLB on the proliferation of LLC1 cells and established a Lewis lung carcinoma model to evaluate the efficacy and biosafety of PLB in the treatment of subcutaneous tumor in mice.Results CCK-8 results showed that PLB significantly suppressed the proliferation of LLC1 cells in a time-and dose-dependent manner and its mechanisms were related to apoptosis and ferroptosis.In Lewis lung carcinoma model,PLB treatment group exhibited stronger antitumor effects versus the control group.The H&E results showed that,compared to the control group,there was an increase in cell nucleus rupture and tissue necrosis area with PLB treatment.Conclusion Both the results of cell and animal experiments confirmed that PLB suppressed the growth and proliferation of NSCLC cells,which may be related to cell apoptosis and ferroptosis.This provides a preclinical basis for treating NSCLC with PLB and promotes the development of new drugs.

关 键 词：白花丹素 非小细胞肺癌 铁死亡 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]