西藏沙棘和肋果沙棘叶绿体基因组比较分析及系统发育研究  

Chloroplast Genomes and Phylogenetic Analysis of Hippophae tibetana and Hip-pophae neurocarpa

作　　者：李昕娟 刘丽娥 李鸿燕 李嘉欣 周武 LI Xinjuan;LIU Li'e;LI Hongyan;LI Jiaxin;ZHOU Wu(School of Eco-Environmental Engineering,Qinghai University,Xining,810016)

机构地区：[1]青海大学生态环境工程学院,西宁810016

出　　处：《基因组学与应用生物学》2024年第5期767-787,共21页Genomics and Applied Biology

基　　金：国家自然科学基金地区科学基金项目(32160386)资助。

摘　　要：沙棘属(Hippophae)植物具有丰富的经济价值和生态价值,但对于沙棘属植物的分类和系统发育研究还存在不足和争论。本研究基于西藏沙棘(Hippophae tibetana)和肋果沙棘(Hippophae neurocarpa)的叶绿体基因组进行分析和研究,以探究沙棘属植物在系统发育和分类上的关系。组装后得到的西藏沙棘和肋果沙棘的叶绿体基因组大小分别为155810 bp和156316 bp,二者(G+C)含量相差仅0.02%;经注释后得到的西藏沙棘叶绿体基因组含有131个基因,肋果沙棘叶绿体基因组含有132个基因;二者分别含有246个和247个简单重复序列,41个和36个长重复序列。在对沙棘属叶绿体基因组进行分析后得出6个高可变区,可用于系统发育分析以及分子标记的开发。由系统发育结果可知,西藏沙棘单独为一支,肋果沙棘则与蒙古沙棘(Hippophae rhamnoides subsp.monglica)等沙棘属亚种聚为一支。本研究可为沙棘属植物的分类和系统发育研究奠定基础。Despite the high economic and ecological value of Hippophae,which possesses rich economic and ecological benefits,re-search on the taxonomy and phylogenetics of Hippophae remains insufficient and controversial.This study is based on an examination of the chloroplast genomes of two Hippophae species—Hippophae tibetana and Hippophae neurocarpa—with the aim of investigating their phylogenetic and taxonomic relationships.After sequencing and assembling,the genome sizes of Hippophae tibetana and Hippophae neurocarpa,were determined to be 155810 bp and 156316 bp,respectively.The difference in(G+C)content between the two types was only 0.02 percents.Through annotation,it was revealed that both Hippophae tibetana genome and Hippophae neurocarpa genome contain 131 and 132 genes,along with 246 and 247 simple sequence repeats,and 41 and 36 long sequence repeats,respectively.Six highly variable regions were identified in the chloroplast genome of Hippophae,which can be utilized for phylogenetic analysis and the development of molecular markers.The resulting phylogenetic tree indicates that Hippophae tibetana forms a distinct clade,while Hipp-ophae neurocarpa and subspecies of Hippophae such as Hippophae rhamnoides subsp.monglica are grouped together in another clade.This investigation will serve as a foundation study for understanding the taxonomy and phylogeny of Hippophae.

关 键 词：西藏沙棘 肋果沙棘 叶绿体基因组 系统发育分析 

分 类 号：S793.6[农业科学—林木遗传育种]