罗汉果甜苷促进高脂饮食诱导小鼠的脂质代谢  

Mogroside Promotes Lipid Metabolism in Mus muscolus Induced by High-fat Diet

作　　者：王永浪 韦义荣 韦丽丽[1] 何翠梅 徐慧凌 黄艳娜[1] 蒋钦杨[1] WANG Yonglang;WEI Yirong;WEI Lili;HE Cuimei;XU Huiling;HUANG Yanna;JIANG Qinyang(College of Animal Science and Technology,Guangxi University,Nanning,530004)

机构地区：[1]广西大学动物科学技术学院,南宁530004

出　　处：《基因组学与应用生物学》2024年第5期828-838,共11页Genomics and Applied Biology

基　　金：广西自然科学基金项目(2022GXNSFAA035525);广西大学“大学生创新创业训练计划”项目(S202210593311)共同资助。

摘　　要：为探究罗汉果甜苷对高脂饮食诱导的小鼠(Mus muscolus)生长性能及脂质代谢的影响,实验选取40只初始体重约为14 g的21日龄雄性KM小鼠,随机分为4个处理组(n=10只/组),即对照组(CK)、低浓度甜苷组(LSG)、中浓度甜苷组(MSG)和高浓度甜苷组(HSG)。预实验7 d,正式实验56 d,实验结束后逐只称重并测定血糖浓度,同时采集称量棕色脂肪、皮下脂肪、腹部脂肪和肝脏等组织脏器的重量;选取棕色脂肪、皮下脂肪石蜡切片进行HE染色以及对肝组织石蜡切片进行油红O染色;通过实时定量PCR(real-time quantitative PCR,RT-qPCR)检测UCP1、PRDM16、PGC-1α及脂肪代谢信号通路各相关基因(AMPK、PPARγ、C/EBPα)在CK组和HSG组的棕色脂肪、腹部脂肪和皮下脂肪中的表达情况。结果表明,灌胃100、300、600 mg.kg^(-1).d^(-1)的罗汉果甜苷溶液可降低高脂饮食诱导小鼠的体重、肝重及血糖,并使高脂饮食诱导小鼠棕色脂肪、皮下脂肪和肝脏脂肪的沉积得到改善。RT-qPCR表明,HSG组的棕色脂肪、腹部脂肪和皮下脂肪中白色脂肪棕色化相关基因(UCP1、PRDM16、PGC-1α、AMPK、PPARγ、C/EBPα)的mRNA相对表达量显著上调(P<0.05)。本研究可为罗汉果甜苷及其相关产业的发展提供重要科学依据,为进一步解析罗汉果甜苷调控脂质代谢的分子机制提供理论参考。In order to study the effect of mogroside on growth performance and lipid metabolism in high-fat diet Mus muscolus,forty 21-day-old male KM Mus muscolus(weighing about 14 g)were selected in this experiment,and they were randomly divided into four treat-ment groups(n=10 animals/group),i.e.,control group(CK),low concentration sweet glycoside group(LSG),medium concentra-tion sweet glycoside group(MSG)and high concentration sweet glycoside group(HSG).The preliminary test lasted for 7 days,and the formal test lasted for 56 days.At the end of the test period,the blood glucose concentration was measured after weighing one by one,and the weights of brown adipose tissue,subcutaneous adipose tissue and liver tissues were collected and weighed;The paraffin sec-tions of brown adipose tissue and subcutaneous adipose tissue were stained with HE and the liver tissue paraffin sections were stained with oil red O.The expression of UCP1,PRDM16,PGC-1α,and the genes(AMPK,PPARγ,and C/EBPα)relating to the fat meta-bolism signaling pathway in brown adipose tissue,abdominal adipose tissue,and subcutaneous fat in the CK group and HSG group was detected by real-time quantitative PCR(RT-qPCR).The results showed that the body weight,liver weight and blood glucose of Mus muscolus fed with 100,300,600 mg.kg^(-1).d^(-1) mogroside solution decreased,and the deposition of brown adipose tissue,subcuta-neous adipose tissue and liver adipose tissue in Mus muscolus fed with high-fat diet increased.RT-qPCR showed that the expression of UCP1,PRDM16,PGC-1α,AMPK,PPARγand C/EBPαmRNA in brown adipose tissue,abdominal adipose tissue and subcutaneous adipose tissue significantly increased in HSG group(P<0.05).This study can provide an important scientific basis for the development of mogroside and its rela-ted industries,and provide a theoretical reference for the further analysis of the molecular mechanism of mo-groside in regulating lipid metabolism.

关 键 词：罗汉果甜苷 小鼠 脂质代谢 脂肪类型转化 

分 类 号：R285.5[医药卫生—中药学]