Revealing the specific regulations of nitric oxide on the postharvest ripening and senescence of bitter melon fruit  

作　　者：Hongwei Wang Ling Li Lili Ma Alisdair R.Fernie Anzhen Fu Chunmei Bai Zhaoze Sang Susu Guo Fan Zhang Qing Wang Yanyan Zheng Jinhua Zuo 

机构地区：[1]Key Laboratory of Vegetable Postharvest Processing,Ministry of Agriculture,Beijing Key Laboratory of Fruits and Vegetable Storage and Processing,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)of Ministry of Agriculture,Key Laboratory of Urban Agriculture(North)of Ministry of Agriculture,Beijing Vegetable Research Center,Institute of Agro-Products Processing and Food Nutrition,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China [2]College of Food Science and Biotechnology,Tianjin Agricultural University,Tianjin 300392,China [3]Max Planck Institute of Molecular Plant Physiology,14476 Potsdam Golm,Germany [4]Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing,China

出　　处：《aBIOTECH》2024年第1期29-45,共17页生物技术通报（英文版）

基　　金：supported by the National Key Research and Development Program of China[2022YFD2100105];Special Innovation Abiity Construction Fund of Beiing Academy of Agricultural and Forestry Sciences (20210437);Collaborative Innovation Center of Beijing Academy of Agricultural and Forestry Sciences (201915).

摘　　要：Bitter melon fruit is susceptible to yellowing,softening,and rotting under room-temperature storage conditions,resulting in reduced commercial value.Nitric oxide(NO)is an important signaling molecule and plays a crucial role in regulating the fruit postharvest quality.In this study,we investigated the effects of NO treatment on changes in sensory and firmness of bitter melon fruit during postharvest storage.Moreover,transcriptomic,metabolomic,and proteomic analyses were performed to elucidate the regulatory mechanisms through which No treatment delays the ripening and senescence of bitter melon fruit.Our results show that differentially expressed genes(DEGs)were involved in fruit texture(CSLE,β-Gal,and PME),plant hormone signal transduction(ACS,JAR4,and AUX28),and fruit flavor and aroma(SUS2,LOX,and GDH2).In addition,proteins differentially abundant were associated with fruit texture(PLY,PME,and PGA)and plant hormone signal transduction(PBL15,JAR1,and PYL9).Moreover,No significantly increased the abundance of key enzymes involved in the phenylpropanoid biosynthetic pathway,thus enhancing the disease resistance and alleviating softening of bitter melon fruit.Finally,differential metabolites mainly included phenolic acids,terpenoids,and flavonoids.These results provide a theoretical basis for further studies on the physiological changes associated with postharvest ripening and senescence of bitter melon fruit.

关 键 词：Bitter melon NO treatment POSTHARVEST Ripening and senescence 

分 类 号：S43[农业科学—农业昆虫与害虫防治]