氧化白藜芦醇抗诺卡菌活性及作用机制  

Anti-Nocardia activity and mechanism of oxyresveratrol

作　　者：孙细欢 巫心怡 黄东红[1] Sun Xihuan;Wu Xinyi;Huang Donghong(The Second Affiliated Hospital of Fujian Medical University,Clinical Microbiology Laboratory,Quanzhou 362000;Fujian Medical University,School of Medical Technology and Engineering,Fuzhou 350100)

机构地区：[1]福建医科大学附属第二医院,临床微生物实验室,泉州362000 [2]福建医科大学,医学技术与工程学院,福州350100

出　　处：《中国抗生素杂志》2024年第6期634-640,共7页Chinese Journal of Antibiotics

基　　金：2021年度福建省中青年教师教育科研项目(No.JAT210121);医院配套的博士科研启动基金(No.BS202002)。

摘　　要：目的探究氧化白藜芦醇(oxyresveratrol,OXY)的抗诺卡菌活性,明确其抗菌效果,同时初步探索其抑制诺卡菌的机制,为筛选新型抗诺卡菌药物提供更多理论基础。方法借鉴纸片扩散法对临床收集的12株诺卡菌进行OXY抗菌活性筛选;通过测定OXY对诺卡菌的MIC值和MBC值,明确其抗诺卡菌活性;通过测定A620的方法,评价OXY对诺卡菌的生长的影响;采用光学显微镜、扫描电子显微镜和透射电子显微镜观察的方法,明确OXY对诺卡菌形态结构变化的影响;最后通过检测培养液上清中碱性磷酸酶(alkaline phosphatase,AKP)和钾离子的含量探索OXY对诺卡菌细胞壁和细胞膜完整性的影响。结果通过借鉴纸片扩散法的筛选发现25和50 mg/mL OXY都能较好地抑制12株诺卡菌的生长,12.5 mg/mL OXY能一定程度上抑制诺卡菌的生长;OXY对12株诺卡菌的MIC值和MBC值分别为0.2和0.4mg/mL;A620的结果表明0.5×MIC OXY和1×MIC OXY都能显著抑制诺卡菌的生长;OXY作用使圣乔治教堂诺卡菌形态发生显著改变,细菌皱缩、变圆、细胞壁变粗糙,细胞发生破裂;OXY还能破坏诺卡菌细胞壁和细胞膜的完整性,上清液中AKP和钾离子含量随着OXY作用浓度的升高而升高。结论OXY具有较好抗诺卡菌活性,其MIC值和MBC值分别为0.2和0.4 mg/mL,OXY可能是通过破坏诺卡菌细胞壁和细胞膜的完整性发挥抗菌作用。Objective To explore the anti-Nocardia activity of oxyresveratrol(OXY),clarify its antibacterial effect,and initially explore the mechanism of its inhibition of Nocardia,so as to provide a more theoretical basis for screening new anti-nocardia drugs.Methods The OXY antibacterial activity against 12 strains of Nocardia was screened by the disk diffusion method.The minimal inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) of OXY against Nocardia were determined to clarify its anti-Nocardia activity.The effect of OXY on the growth of Nocardia was evaluated by the determination of A_(620).The effects of OXY on the morphology and structure of Nocardia were investigated by the optical microscope,the scanning electron microscope and the transmission electron microscope.Finally,the contents of alkaline phosphatase(AKP) and K+ in the supernatant of the culture medium were detected to explore the effect of OXY on the integrity of Nocardia cell walls and membranes.Results By using disk diffusion method,it was found that both 25 and 50 mg/mL OXY could inhibit the growth of 12 strains of Nocardia,and 12.5 mg/mL OXY could inhibit the growth of Nocardia to some extent.The MIC and MBC values of OXY against 12 strains of Nocardia were 0.2 and 0.4 mg/mL,respectively.The results of A_(620) showed that both 0.5×MIC OXY and 1×MIC OXY could significantly inhibit the growth of Nocardia.OXY significantly changed the morphology of N.cyriacigeorgica;the bacteria shrank,became round,the cell wall became rough,and the cells broke down.OXY could also destroy the integrity of the Nocardia cell wall and membrane,and the content of AKP and K+ in the supernatant increased with the increase of the OXY concentration.Conclusion OXY had good antibacterial activity against Nocardia,and its MIC and MBC values were 0.2 and 0.4 mg/mL,respectively.OXY might exert an antibacterial effect by destroying the integrity of the Nocardia cell wall and membrane.

关 键 词：氧化白藜芦醇 诺卡菌 抗菌活性 抗菌机制 

分 类 号：R987.1[医药卫生—药品]