SIRT2、P65和Survivin在乳腺癌中的表达及其与临床病理特征的关系  

Expression of SIRT2,P65,and Survivin in breast cancer and their association with clinicopathological features

作　　者：梅思思 田世维 段彦林 金柔 秦田 周增贵 杨宇石 杨冰清 尹明英 徐澍 MEI Sisi;TIAN Shiwei;DUAN Yanlin;JIN Rou;QIN Tian;ZHOU Zenggui;YANG Yushi;YANG Bingqing;YIN Mingying;XU Shu(Department of Pathology,the Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China;School of Clinical Medicine,Guizhou Medical University,Guiyang 550004,Guizhou,China;Department of Pathology,Anshun People's Hospital,Anshun 561000,Guizhou,China)

机构地区：[1]贵州医科大学附属医院病理科,贵州贵阳550004 [2]贵州医科大学临床医学院,贵州贵阳550004 [3]安顺市人民医院病理科,贵州安顺561000

出　　处：《贵州医科大学学报》2024年第6期819-825,共7页Journal of Guizhou Medical University

基　　金：贵州省卫生计生委科学技术基金(gzwjkj2018-1-062)。

摘　　要：目的通过检测SIRT2、P65和生存素(Survivin)蛋白在乳腺癌中的表达,探讨其在乳腺癌中的相互关系及与乳腺癌的临床病理特征的关系。方法384例乳腺浸润性癌患者作为乳腺癌组,31例乳腺纤维腺病患者作为对照组,采用免疫组化EnVision二步法检测两组乳腺病变组织内SIRT2、P65、Survivin的蛋白表达,同时收集乳腺癌组患者相关临床病理特征资料(年龄、分子分型、TNM分期、组织学分级、淋巴结转移及月经情况),分析SIRT2、P65及Survivin蛋白在乳腺癌组中的表达与临床病理特征的关系,分析SIRT2、P65及Survivin蛋白在乳腺癌组表达的相关性。结果乳腺癌组中SIRT2、Survivin蛋白高表达,表达率均高于对照组,差异具有统计学意义(P<0.05);SIRT2、P65及Survivin蛋白在不同分子分型、TNM分期乳腺癌患者的高表达,差异有统计学意义(P<0.05);SIRT2蛋白在LuminalB1型中的高表达率高于其他分型(P<0.05),P65蛋白在LuminalA型中的高表达率高于其他分型(P<0.05),Survivin蛋白在HER-2阳性型的高表达率高于其他分型(P<0.05);SIRT2、P65及Survivin蛋白在TNM分期中Ⅲ~Ⅳ期高表达率较Ⅰ~Ⅱ期高(P<0.05);SIRT2和P65蛋白在淋巴结转移与非转移乳腺癌患者间的高表达,差异有统计学意义(P<0.05),其中转移的高表达率较非转移高;P65和Survivin蛋白在不同组织学分级乳腺癌患者间高表达,差异有统计学意义(P<0.05),其中Ⅰ级的高表达率较Ⅱ、Ⅲ级高;乳腺癌组中SIRT2与P65和Survivin蛋白表达均呈正相关(r分别为0.371和0.310,P<0.001),P65与Survivin蛋白表达呈正相关(r=0.466,P<0.001)。结论乳腺癌组织存在SIRT2及Survivin蛋白表达上调,这可能是影响乳腺癌组织学分级及TNM分期、分子分型等临床病理特征的机制。Objective To investigate the relationship between SIRT2,P65,and Survivin in breast cancer by detecting the expressions of SIRT2,P65,and Survivin,and explore their association with clinicopathological features.Methods A total of 384 patients with invasive breast cancer were selected as breast cancer group and 31 patients with fibroadenosis as control group.Immuno-histochemical EnVision two-step method was used to detect the protein expressions of SIRT2,P65,and Survivin in breast lesions in two groups.At the same time,clinicopathological features-related information of breast cancer group was collected,including age,molecular typing,TNM staging,histological grading,lymph node metastasis and menstruation.The association of SIRT2,P65,and Survivin protein expressions in breast cancer group with clinicopathological features were analyzed.The correlation between SIRT2,P65,and Survivin protein expressions in breast cancer group were analyzed.Results SIRT2 and Survivin proteins were highly expressed in breast cancer tissues.The rates of highly expressed SIRT2 and Survivin was higher in breast cancer group than those in control group(P<0.05).The protein expressions of SIRT2,P65,and Survivin in different molecular types and TNM stages were higher in breast cancer group than those in control group(P<0.05).The rate of highly expressed SIRT2 protein was higher in Luminal B1 than that in other types,while the rate of highly expressed P65 protein was higher in Luminal A than that in other types.The rate of highly expressed SIRT2 protein was higher in HER2+breast cancer than that in other types,while the rates of highly expressed SIRT2,P65,and Survivin proteins were higher in TNMⅢ~Ⅳ than those in TNMⅠ-Ⅱ.SIRT2 and P65 protein expressions were higher in the patients with lymph node metastasis of breast cancer than those in the patients with non-lymph node metastasis(P<0.05),and SIRT2-and P65-positive rates of the samples with their high expressions were higher than those in SIRT2-and P65-negative rates(P<0.05).P65 and Survi

关 键 词：乳腺肿瘤 SIRT2蛋白 P65蛋白 生存素蛋白 细胞增殖 信号通路 

分 类 号：R737.9[医药卫生—肿瘤]