振心方对压力负荷性心力衰竭大鼠心功能、心室重构及能量代谢的影响  

Effects of Zhenxin prescription on cardiac function,ventricular remodeling and energy metabolism in rats with pressure-load heart failure

作　　者：李淑淳 周莉 朱灵妍 吴琼 刘晨阳 曹敏[1] LI Shuchun;ZHOU Li;ZHU Lingyan;WU Qiong;LIU Chenyang;CAO Min(Department of Emergency,Longhua Hospital Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Department of Traditional Chinese Medicine,Nanxiang Hospital,Jiading District,Shanghai 201802,China;Department of Traditional Chinese Medicine,Shanghai Xuhui District Central Hospital,Shanghai 200031,China)

机构地区：[1]上海中医药大学附属龙华医院急诊科,上海200032 [2]上海市嘉定区南翔医院中医科,上海201802 [3]上海市徐汇区中心医院中医科,上海200031

出　　处：《世界临床药物》2024年第6期597-602,共6页World Clinical Drug

基　　金：国家自然科学基金(82374397);第五批全国中医临床优秀人才研修项目[国中医药人教函(2022)1号];2020年度上海市中西医结合专项(ZHYY-ZXY JHZX-202012);上海中医药大学医养结合高水平科创项目(602075D)。

摘　　要：目的探讨振心方对腹主动脉缩窄心力衰竭(heart failure,HF)大鼠心功能的影响。方法将40只Wistar大鼠随机分为假手术组、模型组、振心方组以及比索洛尔组,每组10只。除假手术组外,其余各组采用腹主动脉缩窄法建立HF大鼠模型,振心方组予振心方混悬液5.6 g/kg/d灌胃,比索洛尔组给予富马酸比索洛尔混悬液1 mg/kg/d灌胃,假手术组与模型组给予等量生理盐水灌胃,连续12周。比较各组大鼠超声心动图、左心室重量指数(left ventricular mass index,LVMI)、脑利钠肽(brain natriuretic peptide,BNP)、醛固酮(aldosterone,ALD)、血管紧张素(angiotensin,Ang)Ⅱ、乳酸、丙酮酸以及游离脂肪酸含量。结果与假手术组相比,模型组血清BNP、ALD、AngⅡ、乳酸、丙酮酸以及游离脂肪酸含量显著上升(P<0.01)。与模型组比较,振心方能升高左室射血分数,改善心肌组织病变、心肌纤维化和LVMI,同时使血清BNP、ALD、AngⅡ、丙酮酸以及游离脂肪酸含量明显下降(P<0.01)。结论振心方能有效干预HF大鼠心功能,改善心室重构,推测可能与减少血清代谢产物堆积有关。Objective To investigate the effect of Zhenxin prescription on cardiac function in rats with abdominal aortic coarctation and heart failure(HF).Method Forty Wistar rats were randomly divided into sham operation group,model group,Zhenxin prescription group and bisoprolol group,with 10 rats in each group.Except the sham operation group,the other groups of rats were treated with abdominal aortic coarctation to establish the HF rat model.Zhenxin prescription group was given 5.6 g/kg/d of Zhenxin prescription suspension,bisoprolol group was given 1 mg/kg/d of bisoprolol fumarate suspension,sham operation group and model group were given the same amount of normal saline intragastric administration for 12 weeks.The contents of echocardiography,left ventricular mass index(LVMI),brain natriuretic peptide(BNP),aldosterone(ALD),angiotensin(Ang)Ⅱ,lactic acid,pyruvic and free fatty acid in all groups were compared.Results Compared with sham operation group,the serum contents of BNP,ALD,AngⅡ,lactic acid,pyruvate and free fatty acid in model group were significantly increased(P<0.01).Compared with model group,Zhenxin prescription could increase left ventricular ejection fraction,improve myocardial tissue lesion,myocardial fibrosis and LVMI,and significantly decrease serum BNP,ALD,AngⅡ,pyruvate and free fatty acid(P<0.01).Conclusion Zhenxin prescription can effectively regulate heart function and improve ventricular remodeling in rats with HF,which may be related to reducing the accumulation of serum metabolites.

关 键 词：振心方 压力负荷性心力衰竭 心功能 心室重构 能量代谢 

分 类 号：R-33[医药卫生] R285.5