滇山茶狮子头及其芽变品种大玛瑙间花色差异分析  

Analysis of Flower Color Difference Between Camellia reticulata‘Shizitou’and its Bud Mutant‘Damanao’

作　　者：周麟 雍清青 姚响 陈晓涓 黄顺满 屈燕[1] ZHOU Lin;YONG Qingqing;YAO Xiang;CHEN Xiaojuan;HUANG Shunman;QU Yan(College of Landscape and Horticulture,Southwest Forestry University/Southwest Research Center for Engineering Technology of Landscape Architecture under National Forestry and Grassland Administration/Yunnan Engineering Research Center for Functional Flower Resources and Industrialization,Kunming 650224;Kunming Greening Engineering Quality Detecting Test Station,Kunming 650224)

机构地区：[1]西南林业大学园林园艺学院/国家林业和草原局西南风景园林工程技术研究中心/云南省功能性花卉资源及产业化技术工程研究中心,昆明650224 [2]昆明市绿化工程质量检测站,昆明650224

出　　处：《植物遗传资源学报》2024年第4期600-611,共12页Journal of Plant Genetic Resources

基　　金：国家“十三五”重点研发计划项目(2019YFD1001005);云南省万人计划青年拔尖人才项目(YNWR-QNBJ-20190211)。

摘　　要：滇山茶是世界著名观赏花木,花色是其重要的观赏性状。滇山茶狮子头花色为深红色,而其芽变品种大玛瑙是滇山茶中唯一红白双色的名贵品种,极具观赏价值。以上述2个品种为研究材料,采用RHSCC比色卡比色法和色差仪测定2个品种滇山茶花蕾期和盛花期花瓣的花色表型,并基于转录组与代谢组分析挖掘呈色相关的关键代谢物及关键基因。花青素靶向代谢分析表明,在滇山茶2个品种中共鉴定出28种花青素代谢物,其中狮子头与大玛瑙红色区域花瓣间没有差异代谢物,狮子头与大玛瑙白色区域花瓣间的关键差异代谢物为矢车菊素-3-O-桑布双糖苷、原花青素B2、原花青素B3、阿福豆苷,大玛瑙花瓣的红白区域关键差异代谢物为矢车菊素-3-O-桑布双糖苷、原花青素B2、阿福豆苷。转录组KEGG分析结果表明,苯丙醇生物合成和类黄酮生物合成途径与大玛瑙红白双色花瓣的形成有关;植物激素信号转导和昼夜节律-植物途径与滇山茶花色芽变有关。转录代谢联合分析共筛选出与滇山茶呈色高度相关的差异表达基因共17条,包括4条CHS、3条HCT、2条F3′H、1条LAR、5条MYB和2条b HLH。本研究结果对进一步揭示花色芽变育种具有一定的参考意义。Camellia reticulata is a famous ornamental flower in the world,and its flower color is an important ornamental character.The flower color of Camellia reticulata‘Shizitou’is deep red,while its bud mutant‘Damanao’showing white-red mixed flower is rare with great ornamental value.In this study,both varieties were analyzed at the flower traits by RHSCC colorimetric method,and their metabolites and transcriptional profiles were examined based on transcriptome and metabolome methodologies.Anthocyanin targeted metabolic analysis detected 28 anthocyanin metabolites.There were no different metabolites between the petals of‘Shizitou’and‘Damanao’in the red region,while the main different metabolites between the white region of the petals of‘Shizitou’and‘Damanao’were centaurin-3-O-sambudigoside,proanthocyanidin B2,proanthocyanidin B3 and afotoside.The main different metabolites between white and red region of‘Damanao’petals were centaurin-3-O-sambuloside,proanthocyanidin B2 and afoside.Transcriptomic KEGG analysis showed that the genes in phenylpropanol biosynthesis and flavonoid biosynthesis pathways were related to the formation of red and white bicolor petals of‘Damanao’.Plant hormone signal transduction and circadian rhythm-plant pathway were associated with flower color bud change of Camellia reticulata.Transcriptional and metabolic joint analysis identified 17 differentially expressed genes highly related to the color of Camellia reticulata,including 4 CHS,3 HCT,2 F3'H,1 LAR,5 MYB and 2 bHLH.This study provided a reference for future breeding based on the flower color bud mutation.

关 键 词：滇山茶 花青素苷 花色 代谢组 转录组 

分 类 号：S685.14[农业科学—观赏园艺]