SPE-HPLC-MS/MS法测定不同类型土壤中磺胺类抗生素  

Determination of Sulfonamides in Diff erent Types of Soil by SPE-HPLC-MS/MS Method

作　　者：史京转 王菀琼 史颖娟 Shi Jingzhuan;Wang Wanqiong;Shi Yingjuan(School of Chemical&Environmental Science,Shaanxi University of Technology,Shaanxi,723001;Shaanxi Reconnaissance Design&Research Institute of Water Environmental Engineering,Shaanxi,710021)

机构地区：[1]陕西理工大学化学与环境科学学院,陕西723001 [2]陕西水环境工程勘测设计研究院,陕西710021

出　　处：《当代化工研究》2024年第12期62-64,共3页Modern Chemical Research

基　　金：2021年陕西省教育厅重点实验室项目“赤泥催化降解典型有机污染物的机理研究”(项目编号:21JS001);2023年陕西省自然科学基础研究项目“赤泥基催化降解利巴韦林的研究”(项目编号:2023-JC-QN-0121);2021年陕西理工大学人才引进科研启动专项“改性赤泥的制备及其去除有机污染物的性能研究”(项目编号:SLGRCQD2112)。

摘　　要：研究了高效液相色谱(HPLC)与固相萃取(SPE)相结合测定不同土壤中磺胺类抗生素(SAs)的分析方法,检测出关中地区不同类型土壤中3种磺胺类抗生素的含量,为进一步研究土壤中磺胺类药物的污染特性提供依据。土样采用V(甲醇):V(缓冲液)=1:1比例进行抗生素的提取;以HLB固相萃取柱净化富集;采用高效液相色谱,以乙腈和0.01 mol/L H_(3)PH_(4)作为流动相,于270 nm波长处对土样进行检测。检测结果表明,不同类型土壤中磺胺类抗生素的总含量在2.75~61.75μg/kg之间。因此,该方法能精确检测出环境中抗生素的含量。The analysis method of high performance liquid chromatography(HPLC)and solid phase extraction(SPE)was studied to determine the content of three sulfonamides(SAs)in different soil types in Guanzhong area,which provided a basis for further research on the pollution characteristics of sulfonamides in soil.The soil sample was extracted with V(methanol):V(mcllvain buffer containing EDTA)=1:1 solution.HLB solid phase extraction column was used for purification and enrichment.The samples were detected by high performance liquid chromatography(HPLC)with acetonitrile and 0.01 mol/L H_(3)PH_(4) as mobile phase at 270 nm.The results showed that the total content of sulfonamides in different types of soil ranged from 2.75~61.75μg/kg.Therefore,the method can accurately detect the amount of antibiotics in the environment.

关 键 词：高效液相色谱 固相萃取 磺胺类抗生素 土壤 

分 类 号：X131.2[环境科学与工程—环境科学]