揭示下一代高通量测序在传染病诊断中的潜力:挑战与前景  

Unraveling the potential of metagenomic next-generation sequencing in infectious disease diagnosis:Challenges and prospects

作　　者：陈宏斌 詹明华 刘思 Francois Balloux 王辉 Hongbin Chen;Minghua Zhan;Si Liu;Francois Balloux;Hui Wang(Department of Clinical Laboratory,Peking University People’s Hospital,Beijing 100044,China;UCL Genetics Institute,University College London,London WC1E 6BT,UK)

机构地区：[1]Department of Clinical Laboratory,Peking University People’s Hospital,Beijing 100044,China [2]UCL Genetics Institute,University College London,London WC1E 6BT,UK

出　　处：《Science Bulletin》2024年第11期1586-1589,共4页科学通报（英文版）

基　　金：supported by the National Key Research and Development Program of China (2022YFA1304300,2018YFE0102100);the National Natural Science Foundation of China (81625014);Beijing Municipal Science and Technology Commission Program (Z191100006619100)。

摘　　要：Pathogen identification for infectious disease diagnosis is crucial and can be achieved through direct isolation of microbes or indirect detection based on the host immune system. Clinical metagenomic next-generation sequencing(mNGS) is an emerging technology that comprehensively analyzes genetic material(DNA and RNA) from both microbes and the host. It does not rely on prior knowledge of a pathogen's genome. This article reviews the clinical utility of mNGS in diagnosing infectious diseases and current technical difficulties.

关 键 词：传染病诊断 DIAGNOSIS CLINICAL 

分 类 号：R181[医药卫生—流行病学]