A subunit vaccine based on Brucella rBP26 induces Th1 immune responses and M1 macrophage activation  

作　　者：Jia Wen Zihua Li Yongxue Lv Shuqin Ding Yazhou Zhu Jihui Yang Jing Tang Mingxing Zhu Yinqi Zhao Wei Zhao 

机构地区：[1]School of Basic Medicine,Ningxia Medical University,Yinchuan 750004,China [2]General Medicine Department,General Hospital of Ningxia Medical University,Yinchuan 750004,China [3]Ningxia Key Laboratory of Prevention and Control of Common Infectious Diseases,Ningxia Hui Autonomous Region,Yinchuan 750004,China

出　　处：《Acta Biochimica et Biophysica Sinica》2024年第6期879-891,共13页生物化学与生物物理学报（英文版）

基　　金：supported by the grant from the Natural Science Foundation of Ningxia Hui Autonomous Region(No.2023AAC03671).

摘　　要：Brucellosis is a global zoonotic infection caused by Brucella bacteria,which poses a significant burden on society.While transmission prevention is currently the most effective method,the absence of a licenced vaccine for humans necessitates the urgent development of a safe and effective vaccine.Recombinant protein-based subunit vaccines are considered promising options,and in this study,the Brucella BP26 protein is expressed using prokaryotic expression systems.The immune responses are evaluated using the well-established adjuvant CpG-ODN.The results demonstrate that rBP26 supplemented with a CpG adjuvant induces M1 macrophage polarization and stimulates cellular immune responses mediated by Th1 cells and CD8+T cells.Additionally,it generates high levels of rBP26-specific antibodies in immunized mice.Furthermore,rBP26 immunization activates,proliferates,and produces cytokines in T lymphocytes while also maintaining immune memory for an extended period of time.These findings shed light on the potential biological function of rBP26,which is crucial for understanding brucellosis pathogenesis.Moreover,rBP26 holds promise as an effective subunit vaccine candidate for use in endemic areas.

关 键 词：BP26 M1 macrophage recombinant protein vaccine Th1 immune responses IMMUNOGENICITY 

分 类 号：R186[医药卫生—流行病学]