Microbiota enterotoxigenic Bacteroides fragilis-secreted BFT-1 promotes breast cancer cell stemness and chemoresistance through its functional receptor NOD1  

作　　者：Wei Ma Lu Zhang Weilong Chen Zhaoxia Chang Juchuanli Tu Yuanyuan Qin Yuwen Yao Mengxue Dong Jiajun Ding Siqin Li Fengkai Li Qiaodan Deng Yifei Yang Tingting Feng Fanrong Zhang Xiying Shao Xueyan He Lixing Zhang Guohong Hu Quentin Liu Yi-Zhou Jiang Shu Zhu Zhi Xiao Dan Su Tong Liu Suling Liu 

机构地区：[1]Fudan University Shanghai Cancer Center & Institutes of Biomedical Sciences,State Key Laboratory of Genetic Engineering,Cancer Institutes,Department of Oncology,Key Laboratory of Breast Cancer in Shanghai,The Shanghai Key Laboratory of Medical Epigenetics,Shanghai Key Laboratory of Radiation Oncology,The International Co-laboratory of Medical Epigenetics and Metabolism,Ministry of Science and Technology,Shanghai Medical College,Fudan University,Shanghai 200032,China [2]Intelligent Pathology Institute and Department of Pathology,The First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230071,China [3]Department of Thyroid,Breast and Vascular Surgery,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,China [4]Institute of Immunology,CAS Key Laboratory of Innate Immunity and Chronic Disease,School of Basic Medical Sciences,Division of Life Science and Medicine,University of Science and Technology of China,Hefei 230027,China [5]Department of Pathology,The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital),Institute of Basic Medicine and Cancer (IBMC),Chinese Academy of Sciences,Hangzhou 310022,China [6]Department of Breast Surgery,The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital),Institute of Basic Medicine and Cancer (IBMC),Chinese Academy of Sciences,Hangzhou 310022,China [7]Department of Breast Medical Oncology,The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital),Institute of Basic Medicine and Cancer (IBMC),Chinese Academy of Sciences,Hangzhou 310022,China [8]Shanghai Institute of Nutrition and Health,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai 200031,China [9]Sun Yat-sen University Cancer Center,State Key Laboratory of Oncology in South China,Guangzhou 510060,China [10]Department of Breast Surgery,Xiangya Hospital,Changsha 410008,China [11]Department of Breast S

出　　处：《Protein & Cell》2024年第6期419-440,共22页蛋白质与细胞（英文版）

基　　金：supported by National Key Research and Development Program of China(2023YFC2506400,2020YFA0112300);National Natural Science Foundation of China(Grant Nos.82230103,81930075,82073267,82203399,82072903);“Ten Thousand Plan”—National High-Level Talents Special Support Plan WR-YK5202101;Program of Shanghai Academic/Technology Research Leader 20XD1400700;Program for Outstanding Medical Academic Leader in Shanghai(2019LJ04);The innovative research team of high-level local university in Shanghai;The Fudan University Research Foundation(IDH 1340042);The Research Foundation of the Fudan University Shanghai Cancer Center(YJRC1603);Shanghai Anticancer Association EYAS PROJECT(SACA-CY23B07);The Natural Science Foundation of Hunan Province(2020JJ4916).

摘　　要：Tumor-resident microbiota in breast cancer promotes cancer initiation and malignant progression.However,targeting microbiota to improve the effects of breast cancer therapy has not been investigated in detail.Here,we evaluated the microbiota composition of breast tumors and found that enterotoxigenic Bacteroides fragilis(ETBF)was highly enriched in the tumors of patients who did not respond to taxane-based neoadjuvant chemotherapy.ETBF,albeit at low biomass,secreted the toxic protein BFT-1 to promote breast cancer cell stemness and chemoresistance.Mechanistic studies showed that BFT-1 directly bound to NOD1 and stabilized NOD1 protein.NOD1 was highly expressed on ALDH+breast cancer stem cells(BCSCs)and cooperated with GAK to phosphorylate NUMB and promote its lysosomal degradation,thereby activating the NOTCH1-HEY1 signaling pathway to increase BCSCs.NOD1 inhibition and ETBF clearance increase the chemosensitivity of breast cancer by impairing BCSCs.

关 键 词：NOD1 breast chemotherapy 

分 类 号：R737.9[医药卫生—肿瘤]