消化性溃疡患者HMGB1、PINP和CD_(4)^(+)T细胞变化及与Hp感染的关系  

Changes of HMGB1,PINP and CD_(4)^(+)T cells in patients with peptic ulcer and their relationship with Hp

作　　者：刘旭 管小倩 郝迁 张彦[2] 齐红兵[1] LIU Xu;GUAN Xiaoqian;HAO Qian;ZHANG Yan;QI Hongbing(Department of Endoscopy Center,Baoding First Central Hospital,Baoding 071000,Hebei,China;Department of Gastroenterology,Baoding First Central Hospital,Baoding 071000,Hebei,China;Department of Laboratory Medicine,Baoding First Central Hospital,Baoding 071000,Hebei,China)

机构地区：[1]保定市第一中心医院内镜诊疗中心,河北保定071000 [2]保定市第一中心医院消化内一科,河北保定071000 [3]保定市第一中心医院检验科,河北保定071000

出　　处：《西部医学》2024年第7期1073-1076,共4页Medical Journal of West China

基　　金：保定市科技计划项目(2041ZF075)。

摘　　要：目的探讨消化性溃疡患者高迁移率族蛋白B1(HMGB1)、I型原胶原N端前肽(PINP)和CD_(4)^(+)T细胞变化及与幽门螺杆菌(Hp)感染的关系。方法选择2019年9月—2021年9月我院收治的消化性溃疡患者97例作为研究对象,根据是否Hp感染分为Hp阳性组(n=63)与Hp阴性组(n=34);另选择同期我院健康体检者60例作为对照组。采用酶联免疫吸附法测定HMGB1和PINP水平;采用流式细胞仪测定CD_(4)^(+)T细胞水平。比较消化性溃疡组与对照组HMGB1、PINP和CD_(4)^(+)T细胞水平;不同Hp感染HMGB1、PINP和CD_(4)^(+)T细胞水平;采用Pearson分析Hp感染与HMGB1、PINP和CD_(4)^(+)T细胞相关性;采用多因素Logistic回归分析HMGB1、PINP和CD_(4)^(+)T细胞与Hp感染的关系。结果消化性溃疡组HMGB1水平高于对照组,PINP和CD_(4)^(+)T细胞水平低于对照组(P<0.05)。Hp阳性组HMGB1水平高于Hp阴性组,PINP和CD_(4)^(+)T细胞水平低于Hp阴性组(P<0.05)。经Pearson分析,HMGB1与Hp感染呈线性正相关,PINP和CD_(4)^(+)T细胞与Hp感染呈线性负相关(P<0.05)。经多因素Logistic回归分析HMGB1、PINP和CD_(4)^(+)T细胞为影响Hp感染危险因素。结论消化性溃疡患者HMGB1水平升高,PINP和CD_(4)^(+)T细胞水平下降,且与Hp感染密切相关,为影响Hp感染的危险因素。Objective To investigate the changes of high mobility group protein B1(HMGB1),procollagen type I N-terminal propeptide(PINP)and CD_(4)^(+)T cells in patients with peptic ulcer and their relationship with Helicobacter pylori(Hp)infection.Methods Our hospital peptic ulcer 97 cases from September 2019 to September 2021 were collected as research objects,which were divide into Hp positive group(n=63)and Hp negative group(n=34)according to whether Hp infection occurs.The 60 cases of physical examination in our hospital from September 2019 to September 2021 were selected as the control group.The levels of serum HMGB1 and PINP were measured by enzyme-linked immunosorbent assay;the CD_(4)^(+)T cells by flow cytometry.The levels of HMGB1,PINP and CD_(4)^(+)T cells in peptic ulcer group and control group were compared;The levels of HMGB1,PINP and CD_(4)^(+)T cells infected with different Hp were compared.Pearson was used to analyze the correlation between Hp infection and HMGB1,PINP and CD_(4)^(+)T cells;multivariate logistic regression was used to analyze the relationship between HMGB1,PINP,CD_(4)^(+)T cells and Hp infection.Results The peptic ulcer group levels of HMGB1 were higher than control group,while the levels of PINP and CD_(4)^(+)T cells was lower than control group(P<0.05).The Hp positive group levels of HMGB1 were higher than Hp negative group,while the levels of PINP and CD_(4)^(+)T cells was lower than Hp negative group(P<0.05).Pearson analysis showed that HMGB1 were linearly positively correlated with Hp negative,while PINP and CD_(4)^(+)T cells were linearly negatively correlated with Hp infection(P<0.05).Multivariate logistic regression analysis showed that HMGB1,PINP and CD_(4)^(+)T cells were risk factors for Hp infection.Conclusion The expression of HMGB1 increases in patients with peptic ulcer,while the level of PINP and CD_(4)^(+)T cells decreases,which is closely related to Hp infection,and is a risk factor for Hp infection.

关 键 词：消化性溃疡 高迁移率族蛋白B1 I型原胶原N端前肽 CD4+T细胞 幽门螺杆菌 

分 类 号：R573.1[医药卫生—消化系统]