牛磺酸抑制心肌纤维化作用  

Inhibitory Effect of Taurine on Myocardial Fibrosis

作　　者：鞠明 乔超峰 许熠 李枫[1] 刘柠 刘洋[1] 王立英[1] JU Ming;QIAO Chaofeng;XU Yi;LI Feng;LIU Ning;LIU Yang;WANG Liying(Basic Medicine College of Beihua University,Jilin 132013,China)

机构地区：[1]北华大学基础医学院,吉林吉林132013

出　　处：《北华大学学报（自然科学版）》2024年第5期615-619,共5页Journal of Beihua University（Natural Science）

基　　金：吉林省科技发展计划项目(YDZJ202401015ZYTS);吉林省教育厅科学技术研究项目(JJKH20220067KJ,JJKH20210057KJ);吉林省卫生健康技术创新项目(2021JC081);北华大学大学生创新创业训练项目(202310201037,202310201040,202310201103)。

摘　　要：目的探讨牛磺酸(Tau)对异丙肾上腺素(Iso)诱导大鼠心肌纤维化(MF)的作用,并阐明其可能的作用机制。方法Wistar大鼠50只,体质量200~250 g,雌、雄各半。随机将Wistar大鼠分为对照组,模型组(Iso组),Tau低(60 mg/(kg·d))、中(120 mg/(kg·d))、高(240 mg/(kg·d))剂量组,每组10只。建立Iso大鼠MF模型,即大鼠背部注射Iso5 mg/(kg·d),连续10 d;对照组注射等体积生理盐水;Tau各剂量组于造模的第2天开始腹腔注射(Tau)给药,1次/d,连续14 d。计算各组大鼠心脏质量指数;HE染色观察心肌组织病理学改变;Masson染色法计算心肌组织胶原百分比;qRT-PCR检测心肌组织中Ⅰ型、Ⅲ型胶原蛋白及结缔组织生长因子(CTGF)mRNA表达。结果Iso组大鼠心脏质量指数显著高于对照组(P<0.05);低、中和高剂量Tau组与模型组比较大鼠心脏质量指数显著降低(P<0.05),且具有浓度依赖性。HE染色结果显示:Iso组大鼠心肌组织中出现典型的MF病理变化;低、中和高剂量Tau组大鼠上述病理变化程度显著改善,且与浓度具有正相关关系。与对照组比较,Iso组大鼠心肌胶原百分比显著高于对照组(P<0.05);与Iso组比较,低、中和高剂量Tau能够显著降低大鼠心肌胶原蛋白百分比(P<0.05)。qRT-PCR结果显示,Iso组大鼠心肌组织中Ⅰ型、Ⅲ型胶原蛋白及CTGF mRNA表达水平显著高于对照组(P<0.01);与Iso组比较,低、中和高剂量Tau能够明显降低Ⅰ型、Ⅲ型胶原蛋白及CTGF mRNA表达水平(P<0.05)。结论Tau可显著降低大鼠心脏质量指数,改善各组大鼠心脏组织病变程度,降低心肌组织中Ⅰ型、Ⅲ型胶原蛋白及CTGF mRNA表达水平,具有抑制大鼠MF的作用。Objective To investigate the effect of Taurine(Tau)on isoproterenol(Iso)induced myocardial fibrosis(MF)in rats and elucidate its possible mechanism.Method Fifty Wistar rats,weighing 200~250 g and half male and half female,were randomly divided into control group,model group(Iso group),low Tau(60 mg/(kg·d)),medium Tau(120 mg/(kg·d))and high Tau(240 mg/(kg·d))dose groups,with 10 rats in each group.The Iso rat MF model was established,the rats were injected with Iso 5 mg/(kg·d)in the back for 10 consecutive days.The control group was injected with normal saline of equal volume.Tau dose groups were intraperitoneally injected with Tau once a day from the 2nd day of modeling for 14 consecutive days.The heart mass index of each group was calculated.Myocardial histopathological changes were observed by HE staining.The collagen percentage of myocardial tissue was calculated by Masson staining.The mRNA expression of typeⅠ,typeⅢcollagen and connective tissue growth factor(CTGF)in myocardial tissue was detected by qRT-PCR.Results The heart mass index in Iso group was significantly higher than that in control group(P<0.05).Compared with the model group,the heart mass index of rats in low,medium and high dose Tau groups was significantly decreased(P<0.05),which was concentration-dependent.HE staining showed that there were typical MF pathological changes in myocardial tissue of Iso group.The degree of the above pathological changes was significantly improved in the low,medium and high dose Tau group,and had a positive correlation with the concentration.Compared with control group,the percentage of myocardial collagen in Iso group was significantly higher than that in control group(P<0.05).Compared with Iso group,low,medium and high doses of Tau groups could significantly reduce the percentage of myocardial collagen in rats(P<0.05).The results of qRT-PCR showed that the expression levels of typeⅠ,typeⅢcollagen and CTGF mRNA in myocardial tissue of Iso group were significantly higher than those of control group(P<0.0

关 键 词：牛磺酸 心脏质量指数 Ⅰ型胶原 Ⅲ型胶原 CTGF 

分 类 号：R285.5[医药卫生—中药学]