微生物发酵合成灵菌红素的研究进展  

Research Progress in Synthesis of Prodigiosin by Microbial Fermentation

作　　者：章文明 羊家辉 姜岷 信丰学 ZHANG Wen-ming;YANG Jia-hui;JIANG Min;XIN Feng-xue(Nanjing Tech University,College of Biological and Pharmaceutical Engineering,State Key Laboratory of Materials-Oriented Chemical Engineering,Nanjing 211816)

机构地区：[1]南京工业大学生物与制药工程学院材料化学工程国家重点实验室,江苏南京211816

出　　处：《微生物学杂志》2024年第3期1-11,共11页Journal of Microbiology

基　　金：国家自然科学基金项目(22078151,22178169);国家重点研发计划项目(2019YFA0905700)。

摘　　要：灵菌红素是一种天然的红色三吡咯色素,主要源自于微生物的次级代谢过程,其具有抗菌、抗肿瘤等功能,在医药、环境、染料等领域具有广阔的应用前景。随着合成生物学技术的不断发展,利用微生物发酵法合成灵菌红素是实现灵菌红素工业化生产的有效途径之一。目前,生产灵菌红素的微生物主要以粘质沙雷氏菌(Serratia marcescens)为主。本文系统介绍了灵菌红素的结构性质和应用潜力,重点阐述了粘质沙雷氏菌中灵菌红素的合成路径,总结了通过高产菌株选育与改造、发酵工艺优化和转录因子调控等策略提高灵菌红素合成的最新研究进展,分析了不同提取纯化工艺的效率,并对未来灵菌红素的高效合成进行了展望,以进一步提高灵菌红素的工业化生产效率。Prodigiosin is a natural red tripyrrole pigment,mainly derived from the secondary metabolic process of microorganisms.It has antibacterial and anti-tumor functions,and has broad application prospects in medicine,environment,dyes and other fields.With the continuous development of synthetic biology technology,the synthesis of prodigiosin by microbial fermentation is one of the effective ways to achieve its industrial production.At present,the microorganisms that produce prodigiosin are mainly Serratia macescernse.Therefore,this paper systematically introduces the structural properties and application potential of prodigiosin,focuses on the synthesis pathway of prodigiosin in Serratia marcescens,and summarizes the latest progress in improving prodigiosin biosynthesis through breeding of high-yielding strains,optimizing fermentation processes,and regulating transcription factors.Moreover,we also analyzes the efficiency of different extraction and purification processes,and looks forward to the highly efficient synthesis of prodigiosin in the future,in order to further improve the industrial production efficiency.

关 键 词：灵菌红素 粘质沙雷氏菌 生物合成 发酵工艺 调控因子 

分 类 号：Q93-3[生物学—微生物学]