汉黄芩素调节磷脂酰肌醇3激酶/丝氨酸苏氨酸蛋白激酶/核因子κB信号通路对慢性阻塞性肺疾病大鼠辅助性T细胞17/调节性T细胞平衡的影响  

Effects of wogonin on regulating phosphatidyl inositol 3 kinase/serine threonine protein kinase/nuclear factorκB signaling pathway on helper T cell 17/regulatory T cell balance in rats with chronic obstructive pulmonary disease

作　　者：尹占良 夏新婷 胡营斌 李泉 冯琦 YIN Zhanliang;XIA Xinting;HU Yingbin;LI Quan;FENG Qi(Pneumology Department,Langfang Fourth People's Hospital,Langfang,Hebei 065700,China;Internal Medicine,Langfang Fourth People's Hospital,Langfang,Hebei 065700,China;Cardiothoracic Surgery,Langfang Fourth People's Hospital,Langfang,Hebei 065700,China)

机构地区：[1]廊坊市第四人民医院呼吸科,河北廊坊065700 [2]廊坊市第四人民医院内科,河北廊坊065700 [3]廊坊市第四人民医院胸心外科,河北廊坊065700

出　　处：《安徽医药》2024年第8期1523-1528,共6页Anhui Medical and Pharmaceutical Journal

基　　金：河北省医学科学研究项目(20211793)。

摘　　要：目的探讨汉黄芩素(Wog)调节磷脂酰肌醇3激酶(PI3K)/丝氨酸苏氨酸蛋白激酶(Akt)/核因子κB(NF-κB)信号通路对慢性阻塞性肺疾病(COPD)大鼠辅助性T细胞17(Th17)/调节性T细胞(Treg)平衡的影响。方法2022年8-12月,大鼠采用随机数字表法分为Model组、低剂量Wog组(Wog-L组,50 mg/kg)、高剂量Wog组(Wog-H组,100 mg/kg)、阳性药物氨茶碱组(Ami组,2.3 mg/kg)、IGF-1(PI3K激活剂)组(1.33 mg/kg)、Wog-H+IGF-1组(100 mg/kg+1.33 mg/kg)、对照组(CK组),每组12只。除CK组外,其他组大鼠均需利用烟熏法联合气管滴注脂多糖(LPS)的方法构建COPD模型,建模成功24 h后,进行给药处理,每天1次给药,持续4周。检测呼气峰流量(PEF)、每分钟通气量(MV)、吸气峰流量(PIF);流式细胞术检测外周血中Th17/Treg;HE染色检测肺组织病理;酶联免疫吸附法检测大鼠肺组织中白细胞介素(IL)-17、IL-10水平;蛋白质印迹法检测肺组织中维甲酸相关孤核受体γt(RORγt)、叉头框蛋白P3(Foxp3)、磷酸化PI3K(p-PI3K)、磷酸化Akt(p-Akt)、磷酸化NF-κB p65(p-NF-κB p65)蛋白。结果与CK组比较,Model组大鼠PEF(12.56±0.47比8.72±0.39)、PIF(9.35±0.32比7.24±0.17)、MV(132.26±5.78比96.63±3.28)、Treg(31.18±2.62比15.52±1.01)比例、IL-10(23.35±1.16比8.85±0.27)明显降低(均P<0.05);Th17(3.14±0.13比18.86±1.67)比例、Th17/Treg(0.10±0.01比1.22±0.11)、肺泡间隔(33.36±1.48比49.78±1.73)、气道炎症评分(0比4.56±0.23)及IL-17(75.83±3.60比185.56±8.62)水平明显升高(均P<0.05)。与Model组比较,Wog-L组、Wog-H组PEF(9.66±0.40,11.49±0.51)、PIF(8.28±0.19,9.03±0.22)、MV(105.54±4.11,126.67±5.72)、Treg(19.93±1.18,27.73±2.05)比例、IL-10(11.56±0.33,20.72±0.59)水平明显升高(均P<0.05);Th17(3.14±0.13比18.86±1.67)比例、Th17/Treg(0.10±0.01比1.22±0.11)、肺泡间隔(43.45±1.26,35.78±1.12)、气道炎症评分(3.75±0.17,0.86±0.07)、IL-17(162.27±7.14,103.35±4.33)水平明显降低(均P<0.05)。与CK组比较,Model组Objective To explore the regulation of wogonin(Wog)on phosphoinositide-3-kinase(PI3K)/serine threonine protein kinase,Effect of Akt/nucler factor-kappa B(NF-κB)signaling pathway on chronic obstructive pulmonary disease,Effects of T helper cell 17(Th17)/regulatory T cell(Treg)balance in COPD rats.Methods From August to December 2022,Rats were randomly grouped into Model group,low-dose Wog group(Wog-L group,50 mg/kg),high-dose Wog group(Wog-H group,100 mg/kg),positive drug aminophylline group(Ami group,2.3 mg/kg),IGF-1(PI3K activator)group(1.33 mg/kg),Wog-H+IGF-1 group(100 mg/kg+1.33 mg/kg),CK group,12 in each group.Except for the CK group,the rats in other groups were used to build COPD models by the method of smoking combined with tracheal instillation of lipopolysaccharide(LPS),24h after the successful modeling,drug treatment was carried out,and the drug was administered once a day for 4 weeks.The changes of peak expiratory flow(PEF),peak inspiratory flow(PIF)and minute ventilation(MV)were detected;flow cytometry detect Th17/Treg in peripheral blood;HE staining detect the pathology of lung tissue;ELISA method was applied to detect the levels of interleukin(IL)-17 and IL-10 in rat lung tissue;Detection of RORγt,Foxp3,phosphorylated PI3K(phosphorylated-PI3K,p-PI3K),phosphorylated Akt(phosphorylated-Akt,p-Akt),phosphorylated NF-κB p65(phosphorylated-NF-κB,p-NF-κB p65)protein in lung by Western blotting.Results Compared with CK group,Model group PEF(12.56±0.47 vs.8.72±0.39),PIF(9.35±0.32 vs.7.24±0.17),MV(132.26±5.78 vs.96.63±3.28),Treg(31.18±2.62 vs.15.52±1.01)proportion and IL-10(23.35±1.16 vs.8.85±0.27)were significantly decreased(all P<0.05);Th17(3.14±0.13 vs.18.86±1.67)ratio,Th17/Treg(0.10±0.01 vs.1.22±0.11),alveolar interval(33.36±1.48 vs.49.78±1.73),airway inflammation score(0 vs.4.56±0.23)and IL-17(75.83±3.60 vs.185.56±8.62)were significantly increased(all P<0.05).Compared with Model group,PEF(9.66±0.40,11.49±0.51),PIF(8.28±0.19,9.03±0.22),MV(105.54±4.11,126.67±5.72),Treg(19.93±

关 键 词：类黄酮物质 慢性阻塞性肺疾病 辅助性T细胞17/调节性T细胞 磷脂酰肌醇3激酶/丝氨酸苏氨酸蛋白激酶/核因子κB信号通路 

分 类 号：R285.5[医药卫生—中药学]