甲硫腺苷磷酸化酶、组蛋白赖氨酸三甲基转移酶蛋白在结直肠癌组织中的表达及临床意义  

Expression and clinical significance of MTAP and SETD2 protein in colorectal cancer

作　　者：周晓萍 汪志翔 ZHOU Xiaoping;WANG Zhixiang(Department of Pathology,Suzhou Hospital of Integrative Medicine,Suzhou,Jiangsu 215101,China;Department of General Surgery,Suzhou High-tech District People's Hospital,Suzhou,Jiangsu 215129,China)

机构地区：[1]苏州市中西医结合医院病理科,江苏苏州215101 [2]苏州高新区人民医院普外科,江苏苏州215129

出　　处：《安徽医药》2024年第8期1537-1540,I0001,共5页Anhui Medical and Pharmaceutical Journal

基　　金：国家青年科学基金资助项目(81803242)。

摘　　要：目的探讨甲硫腺苷磷酸化酶(MTAP)、组蛋白赖氨酸三甲基转移酶(SETD2)蛋白在结直肠癌组织中的表达及临床意义。方法将2018年11月至2019年11月在苏州市中西医结合医院进行手术治疗并经术后病理学检查证实的86例结直肠癌病人作为研究对象,并收集齐其癌组织标本以及对应的癌旁组织标本。采用免疫组织化学染色法检测所有标本MTAP、SETD2蛋白表达水平。采用χ^(2)检验和多因素Cox回归法探讨MTAP、SETD2蛋白表达与结直肠癌病人临床病理特征、预后的关系。结果结直肠癌组织中MTAP、SETD2阳性表达率(30.23%、27.91%)分别低于癌旁组织(58.14%、62.79%)(P<0.05)。MTAP、SETD2阳性表达水平随着TNM分期越晚、分化程度越低、伴有淋巴结转移而降低(P<0.05)。MTAP阳性3年生存率(73.08%)明显高于阴性(43.33%)(P=0.011);SETD2阳性3年生存率(70.83%)高于阴性(45.16%)(P=0.011)。单因素、多因素分析得出,TNMⅢ~Ⅳ期、伴淋巴结转移、低分化、MTAP阴性、SETD2阴性表达均为影响结直肠癌预后的危险因素(P<0.05)。结论直肠癌组织中MTAP、SETD2蛋白均呈低表达,其表达水平可能参与疾病发展,可成为评估病人预后不良的有效生物学指标。Objective To explore the expression and clinical significance of 5'-methylthioadenosine phosphorylase(MTAP)and SET domain containing 2(SETD2)in colorectal cancer.Methods From November 2018 to November 2019,86 patients with colorectal cancer who underwent surgical treatment and were confirmed by postoperative pathological examination were enrolled in this study,the cancer tissue specimens and corresponding para-cancer tissue specimens were collected.The relationship between the MTAP and SETD2 protein and the clinicopathological feature and prognosis of colorectal cancer patients were explored byχ^(2) test and multivariate Cox regression.Results The positive expression rates of MTAP and SETD2 in colorectal cancer tissues(30.23%,27.91%)were lower than those in adjacent tissues(58.14%,62.79%)(P<0.05).The positive expression levels of MTAP and SETD2 decreased with TNM stage,differentiation and lymph node metastasis(P<0.05).The 3-year survival rate of MTAP(73.08%)was significantly higher than that of MTAP(43.33%)(P=0.011)The 3-year survival rate of SETD2 positive patients(70.83%)was higher than that of SETD2 negative patients(45.16%)(P=0.011).Univariate and multivariate analysis showed that TNM StageⅢ-Ⅳ,lymph node metastasis,poor differentiation,negative expression of MTAP and SETD2 were all risk factors affecting the prognosis of colorectal cancer(P<0.05).Conclusion The expression of MTAP and SETD2 protein was low in rectal cancer,which may be involved in the development of the disease and may be an effective biological marker to evaluate the poor prognosis of the patients.

关 键 词：结直肠肿瘤 甲硫腺苷磷酸化酶 组蛋白赖氨酸三甲基转移酶 预后 

分 类 号：R735.34[医药卫生—肿瘤]